JournalofVisualizedExperimentswww.jove.comCopyright©2010JournalofVisualizedExperimentsPage1of1MaterialsListfor:Split-UbiquitinBasedMembraneYeastTwo-Hybrid(MYTH)System:APowerfulToolForIdentifyingProtein-ProteinInteractionsJamieSnider1,2,3,SaranyaKittanakom1,2,3,JasnaCurak1,2,3,IgorStagljar1,2,31DepartmentofBiochemistry,UniversityofToronto2DepartmentofMolecularGenetics,UniversityofToronto3TerrenceDonnellyCentreforCellularandBiomolecularResearch(CCBR),UniversityofTorontoCorrespondenceto:IgorStagljaratigor.stagljar@utoronto.caURL:http://www.jove.com/video/1698DOI:doi:10.3791/1698MaterialsNameCompanyCatalogNumberCommentsPolyethenleneGlycol(PEG3350)BioShopCanadaPEG335LithiumAcetateBihydrateBioShopCanadaLIA001X-Gal(5-Bromo-4-Chloro-3-Indolyl-b-D-galactopyranoside)BioShopCanadaXGA001N`,N-dimethylformamideBioShopCanadaDMF4513-amino-1,2,4-triazole(3-AT)BioShopCanadaATT124SodiumphosphatedibasicBioShopCanadaSPD307SodiumphosphatemonobasicFisherScientificBP329-500SalmonSpermDNAVWRinternationalCA80601-120D-GlucoseBioShopCanadaGLU501LBBrothLENOXBioShopCanadaLBL405YeastNitrogenBaseBioShopCanadaYNB406YeastExtractBioShopCanadaYEX401PeptoneBDBiosciences211677Bio-TryptoneBioShopCanadaTRP402AdenineSulphateBioShopCanadaADS201L-UracilBioShopCanadaURA241L-ThreonineBioShopCanadaTHR002L-HistidineBioShopCanadaHIS200L-MethionineBioShopCanadaMET222L-ValineBioShopCanadaVAL201L-PhenylalanineBioShopCanadaPHA302L-IsoleucineBioShopCanadaISO910L-TyrosineBioShopCanadaTYR333L-LeucineBioShopCanadaLEU222L-ArginineBioShopCanadaARG006L-TryptophaneFisherScientificBP395-100L-LysineBioShopCanadaLYS101L-AlanineFisherScientificBP369-100AgarBioShopCanadaAGR001SodaLimeGalssBeadsBiospecProducts11079105SodiumChlorideBioShopCanadaSLD002
