CyagenBiosciencesInc.www.cyagen.comOriCellTMStrainbalb/cMouseMesenchymalStemCellGrowthMediumCatalogNo.MUCMX-90011ProductDescription:Strainbalb/cMouseMesenchymalStemCellGrowthMediumconsistsofoptimizedStrainbalb/cMouseMesenchymalStemCellBasalMediumandpre-selectedFetalBovineSerum.ThisproducthasbeendevelopedfortheoptimalproliferationofStrainbalb/cMouseMesenchymalStemCells(Cat.No.MUCMX-01001),underwhichconditionStrainbalb/cMouseMesenchymalStemCellscanbeexpandedthroughmultiplepassagesandretaintheabilitytodifferentiateintoosteoblast,adipocyteandchondrocyte.Theproductisintendedforlaboratoryresearchuseonly,notfordrug,household,orotheruses.KitComponents:Strainbalb/cMouseMesenchymalStemCellBasalMedium(Cat.No.MUCMX-03011-440)440mLMesenchymalStemCell-QualifiedFetalBovineSerum(Cat.No.MUCMX-05001-50)50mLPenicillin-Streptomycin5mLGlutamine5mLInstructions:PreparationofCompleteMedium1.Priortouse,thawMesenchymalStemCell-QualifiedFetalBovineSerumunderrefrigeration(2to8°C)overnightoruntilcompletelythawed.Gentlyswirlthebottletoensurehomogeneity.MesenchymalStemCell-QualifiedFetalBovineSerumhasbeenheat-inactivatedandisreadytouseafterthawing.Note:Thethawedserummaycontainsomeflocculentprecipitates.Thepresenceofthesesubstancesinserumdoesnotaltertheperformancecharacteristicsoftheproduct.ItisnotCBPI0087A1MUCMX-900111/3CyagenBiosciencesInc.www.cyagen.comrecommendedtofiltertheserumtoremovetheseprecipitates.Doingsomayresultinthelossofsomeserumnutrients.2.About30minutespriortouse,thawPenicillin-StreptomycinsolutionandGlutaminesolutionatroomtemperature.Gentlyswirlthevialstoensurehomogeneity.3.Disinfectwith70%v/vethanoltheexternalsurfacesofthebottles/vialsforeverycomponentinthekit.Allowethanoltoevaporateaway.4.Inalaminarflowhoodasepticallyopenthebottles/vials.5.TransfertheentireamountofMesenchymalStemCell-QualifiedFetalBovineSerum,Penicillin-StreptomycinsolutionandGlutaminesolutionintoStrainbalb/cMouseMesenchymalStemCellBasalMedium.6.Rinseeachvialwiththemediumandtransfertherinsemediumbacktothebot...
