JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:DevelopmentofCell-typespecificanti-HIVgp120aptamersforsiRNAdeliveryJiehuaZhou1,HaitangLi1,JaneZhang2,SwiderskiPiotr3,JohnRossi11DepartmentofMolecularandCellularBiology,BeckmanResearchInstituteofCityofHope2GraduateSchoolofBiologicalSciences,BeckmanResearchInstituteofCityofHope3SharedResource-DNA/RNAPeptide,BeckmanResearchInstituteofCityofHopeCorrespondenceto:JohnRossiatJrossi@coh.orgURL:http://www.jove.com/video/2954DOI:doi:10.3791/2954MaterialsNameCompanyCatalogNumberCommentsMF-MilliporemembranefilterEMDMilliporeHAWP01300Poresize0.45μmSwinnexFilterholderEMDMilliporeSX000130013mmdiameterQIAquickGelExtractionKitQiagen28706DNApurificationMicroconYM-30columnEMDMillipore42410RNAconcentrationBio-spin30columnBio-Rad732-6250RNApurificationTaqPCRDNApolymeraseSigma-AldrichD1806ThermoScriptRT-PCRsystemInvitrogen11146-024DuraScribeT7transcriptionKitEpicentreBiotechnologiesDS010925dNTPforPCRRocheGroup1581295Ribonucleicacid,transferfromE.coliSigma-AldrichR1753tRNAcompetitorHIV-1Ba-Lgp120proteinNationalInstitutesofHealth4961TargetproteinSilencersiRNAlabelingkit-Cy3Ambion1632Acidphenol/chloroform5/1solution(pH4.5)AmbionAM9720Chloroform/Isopropanol24/1solutionSigma-AldrichC0549Calfintestinalphosphatase(CIP)NewEnglandBiolabsM0290LT4polynucleotidekinaseNewEnglandBiolabsM0201LGlycogenRocheGroup10901393001RNAprecipitationGamma-P32-ATPMPBiomedicals013502002Radiactivity40%AccuGel19:1NationalDiagnosticsEC-85010xTBENationalDiagnosticsEC-860N,N,N,N’-Tetramethylethylenediamine(TMEMD)Sigma-AldrichT9281Ammoniumpersulfate(APS)Sigma-AldrichA3678L-methioinesulfoximineSigma-AldrichM5379-250mgRPMIMedia1640Invitrogen11835-030SodiumBicarbonatesolution,7.5%w/vInvitrogen25080-094MinimumEssentialMedium(MEM)(10')Invitrogen11430-030MEMnon-essentialaminoacid(100')Invitrogen11140-050TAcloningkitwithpCR2.1InvitrogenK2040-01
