JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:AssayingSurfaceExpressionofChemosensoryReceptorsinHeterologousCellsSandeepaDey1,SenmiaoZhan2,HiroakiMatsunami11DepartmentofMolecularGeneticsandMolecularBiology,DukeUniversity2DepartmentofChemistry,DukeUniversityCorrespondenceto:HiroakiMatsunamiatmatsu004@mc.duke.eduURL:http://www.jove.com/video/2405DOI:doi:10.3791/2405MaterialsNameCompanyCatalogNumberCommentsCoverslip22X22mm(#1)ThermoFisherScientific,Inc.72200-11FetalbovineserumGIBCO,byLifeTechnologies16000Lipofectamine2000Invitrogen11668-019MinimalessentialmediumSigma-AldrichM4655WithLglutamate,Earle’ssaltandbicarbonateMowiolCalbiochem475904ParaformaldehydeEMS19208HanksBalancedSaltSolution(HBSS)1XGIBCO,byLifeTechnologies14025WithcalciumchlorideandmagnesiumchlorideHEPESBufferSolutionGIBCO,byLifeTechnologies15630PhosphateBufferSaline(PBS)Cellgro21-040-CVWithoutcalciumandmagnesiumPolyDLysineHydrobromideSigma-AldrichP7280SodiumAzideElectronMicroscopySciencesSX0299-1Tissueculturedish35X10mmFalconBD353801Trypsin-EDTAGIBCO,byLifeTechnologies253000.05%
