JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:MicroRNADetectioninProstateTumorsbyQuantitativeReal-timePCR(qPCR)AidaGordanpour1,RobertK.Nam2,LindaSugar3,StephanieBacopulos1,ArunSeth1,3,41DepartmentofLaboratoryMedicine&Pathobiology,UniversityofToronto2DivisionofUrology,SunnybrookHealthSciencesCentre,Toronto,Canada3DepartmentofAnatomicPathology,SunnybrookHealthSciencesCentre,Toronto,Canada4BiologicalSciences,SunnybrookResearchInstituteCorrespondenceto:ArunSethatArun.Seth@utoronto.caURL:http://www.jove.com/video/3874DOI:doi:10.3791/3874MaterialsNameCompanyCatalogNumberCommentsTRIzolReagentInvitrogen15596miScriptReverseTranscriptionKitQiagen218061miScriptPrimerAssaysQiagenExperimentspecificmiScriptSYBRGreenPCRKitQiagen218073LightCycler3.5Real-TimePCRSystemRocheGroupLightCyclerCapillariesRocheGroup04929292001NanoDrop1000spectrophotometerThermoFisherScientific,Inc.2538Agilent2100BioanalyzerG2943CA