JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:Highefficiency,Site-specificTransfectionofAdherentCellswithsiRNAUsingMicroelectrodeArrays(MEA)ChetanPatel1,JitMuthuswamy11SchoolofBiologicalandHealthSystemsEngineering,ArizonaStateUniversityCorrespondenceto:JitMuthuswamyatjit@asu.eduURL:http://www.jove.com/video/4415DOI:doi:10.3791/4415MaterialsNameCompanyCatalogNumberCommentsCellmedia:AdvancedMEML-Glutamine200mMPenicillin/StreptomycinFetalbovineserumGibco/InvitrogenHimediaLaboratories/VWRLonzagroupLtd.Gibco/Invitrogen12492-01395057-44809-757F16000-044Cellmediacomposition:2%FBS,2%L-glutamineand2%PennstrepinAdvanceMEMTrypsinEDTAMediatech,Inc.25-053-CLPBSMediatech,Inc.21-040-CVAlexa488andrhodaminetaggedscrambledsequencesiRNAQiagen,Inc.1027292ElectroporationbufferBioradLaboratories165-2677WaveformgeneratorPragmatic2414AAnywaveform/pulsegeneratorthatcandeliverthedesiredpulsescanbeused.
