PublicationNumberMAN0007350Rev.1.00CollagenaseDescriptionLifeTechnologies’groupofcollagenaseproductsarepurifiedfromClostridiumhistolyticum.Theyareintendedforcellandtissuedisaggregation.Collagenaseisaproteasewithspecificityforthebondbetweenaneutralaminoacid(X)andglycineinthesequencePro-X-Gly-Pro.Thissequenceisfoundinhighfrequencyincollagen.Collagenaseisuniqueamongproteasesinitsabilitytodegradethetriplehelicalnativecollagenfibrilscommonlyfoundinconnectivetissue.ThecollagenasemostcommonlyusedfortissuedissociationisacrudepreparationcontainingclostripiopeptidaseAandanumberofotherproteases,polysaccharidases,andlipases.Thiscrudepreparationisideallysuitedfortissuedissociationbecauseitcontainstheenzymerequiredtoattacknativecollagenandreticularfibers,inadditiontotheenzymeswhichhydrolyzetheotherproteins,polysaccharides,andlipidsintheextracellularmatrixofconnectiveandepithelialtissues.Crudecollagenasedoesexhibitlot-to-lotvariabilityandmayproduceoccasionaltoxicity.Theactivityofthesecrudecollagenasepreparationshasbeencorrelatedwiththeireffectivenessatdissociatingspecifictissuetypesleadingtotheclassificationofcrudecollagenasepreparationsbytype.Theseselectedtypeshavebeenfoundtogivebetterperformanceinpreparationofcellsfromthevarioustissues(Table1).ProductCatalogNo.AmountStorageCollagenase:TypeITypeIITypeIVCollagenase,lyophilized17100-01717101-01517104-01917018-0291g1g1g500mg2°Cto8°C2°Cto8°C2°Cto8°C2°Cto8°CProductUseForResearchUseOnly.NotforuseindiagnosticproceduresSafetyInformationReadtheSafetyDataSheets(SDSs)andfollowthehandlinginstructions.Wearappropriateprotectiveeyewear,clothing,andgloves.Avoidinhalationandskincontact.UnitDefinitionOneproteaseunitliberates1µmolofL-leucineequivalentsfromcollagenin5hoursat37°C,pH7.5.Table1:ProductSelectionCollagenaseTissue/CelltypeTypeIEpithelial,Adrenal,Lung,FatTypeIIHeart,Thyroid,Salivary,Liver,Bone,CartilageTypeIVIslet(insulinreceptorsites)UseReconstituteCollagenase1.Add1mLHank’sBalancedSaltSolution(HBSS)withcalciumandmagnesiumdi...
