PhalloidinfromAmanitaphalloidesandPhalloidinConjugates(Coumarin,FITC,andTRITC)Phalloidin,CatalogNumberP2141Phalloidin,Coumarinlabeled,CatalogNumberP2495Phalloidin,Fluoresceinisothiocyanatelabeled,CatalogNumberP5282Phalloidin,TetramethylrhodamineBisothiocyanate,CatalogNumberP1951ProductDescriptionHNSNHNHNHNCHHNNHCH2COHCH3CH2OHNHOHH3CCH3OOOOOHOOCH3OPhysicalPropertiesOfPhalloidin(CatalogNumberP2141):MolecularFormula:C35H48N8O11SMolecularWeight:788.9(anhydrous)ExtinctionCoefficient:2E1%=0.597(295nminwater)StoreatRoomTemperaturePhysicalPropertiesOfPhalloidin,Coumarinlabeled(CatalogNumberP2495):MolecularFormula:C62H75N11O15S2MolecularWeight:1278.45Excitation:2384nmEmission:2,3470nmMolarExtinctionCoefficient:410,000(275nminethanol)StorageTemperature–20°CPhysicalPropertiesOfPhalloidin,Fluoresceinisothiocyanatelabeled(CatalogNumberP5282):MolecularFormula:C58H63N10O14S4MolecularWeight:1252.4Excitation:3495nmEmission:3513nmMolarExtinctionCoefficient:370,000(495nm)StorageTemperature–20°CPhysicalPropertiesOfPhalloidin,Tetramethyl-rhodamineBisothiocyanate(TRITC)(CatalogNumberP1951):MolecularFormula:C62H72N12O12S4MolecularWeight:1305.6Excitation:3,5540–545nmEmission:3,5570–573nmMolarExtinctionCoefficient:380,000(545nm)StorageTemperature–20°CPhalloidinisafungaltoxinisolatedfromthepoisonousmushroomAmanitaphalloides.ItstoxicityisattributedtotheabilitytobindFactininliverandmusclecells.Asaresultofbindingphalloidin,actinfilamentsbecomestronglystabilized.Phalloidinhasbeenfoundtobindonlytopolymericandoligomericformsofactin,andnottomonomericactin.Thedissociationconstantoftheactin-phalloidincomplexhasbeendeterminedtobeontheorderof3×10–8M.6Phalloidindiffersfromamanitininrapidityofaction;athighdoselevels,deathofmiceorratsoccurswithin1or2hours.1Fluorescentconjugatesofphalloidinareusedtolabelactinfilamentsforhistologicalapplications.2,3,5–9Somestructuralfeaturesofphalloidinarerequiredforthebindingtoactin.However,thesidechainofaminoacid7(γ-δ-dihydroxyleucine)isaccessibleforchemic...
