BDBiosciences-DiscoveryLabwareForResearchUseOnlyGUIDELINESFORUSEPRODUCT:BDMatrigel™BasementMembraneMatrix,5mlvialCATALOGNUMBER:356234BACKGROUND:Basementmembranesarethinextracellularmatricesunderlyingcellsinvivo.BDMatrigelBasementMembraneMatrixisasolubilizedbasementmembranepreparationextractedfromtheEngelbreth-Holm-Swarm(EHS)mousesarcoma,atumorrichinextracellularmatrixproteins.Itsmajorcomponentislaminin,followedbycollagenIV,heparansulfateproteoglycans,entactin/nidogen.1,2BDMatrigelBasementMembraneMatrixalsocontainsTGF-beta,epidermalgrowthfactor,insulin-likegrowthfactor,fibroblastgrowthfactor,tissueplasminogenactivator,3,4andothergrowthfactorswhichoccurnaturallyintheEHStumor.BDMatrigelBasementMembraneMatrixiseffectivefortheattachmentanddifferentiationofbothnormalandtransformedanchoragedependentepithelioidandothercelltypes.Theseincludeneurons,5,6hepatocytes,7Sertolicells,8,9chicklens,10andvascularendothelialcells.11MatrigelBasementMembraneMatrixwillinfluencegeneexpressioninadultrathepatocytes12,13aswellasthreedimensionalcultureinmouse14-17andhuman18,19mammaryepithelialcells.Itisthebasisforseveraltypesoftumorcellinvasionassays,20,21willsupportinvivoperipheralnerveregeneration,22-24andprovidesthesubstratenecessaryforthestudyofangiogenesisbothinvitro25,26andinvivo.27-29BDMatrigelBasementMembraneMatrixalsosupportsinvivopropagationofhumantumorsinimmunosupressedmice.30-32Forfurtherinformation,pleasevisitourwebsiteatwww.bdbiosciences.com.SOURCE:Engelbreth-Holm-Swarm(EHS)MouseTumorFORMULATION:Dulbecco'sModifiedEagle'sMediumwith50μg/mlgentamycinMatrigelBasementMembraneMatrixiscompatiblewithallculturemediaSTABILITY:Stableforaminimumofthreemonthsfromdayofshipmentwhenstoredat-20°CKEEPFROZENRECONSTITUTIONANDUSE:ColorvariationsmayoccurinfrozenorthawedvialsofBDMatrigelBasementMembraneMatrix,rangingfromstrawyellowtodarkredduetotheinteractionofcarbondioxidewiththebicarbonatebufferandphenolred.Variationincolorisnormal,doesnotaffectproductefficacy,andwilldisappearuponequilibrationw...
