TheOpenBiomedicalEngineeringJournal,2010,4,71-76711874-1207/102010BenthamOpenOpenAccessEffectofBio-Oss®CollagenandCollagenMatrixonBoneFormationR.W.K.Wong*andA.B.M.RabieBiomedicalandTissueEngineering,UniversityofHongKong,PrincePhilipDentalHospital,34HospitalRoad,SaiYingPun,HongKongAbstract:Objective:tocomparetheamountofnewboneproducedbyBio-Oss®Collagentothatproducedbycollagenmatrixinvivo.Method:eighteenbonedefects,5mmby10mmwerecreatedintheparietalboneof9NewZealandWhiterabbits.6defectsweregraftedwithBio-Oss®Collagen.6defectsweregraftedwithcollagenmatrixalone(positivecontrol)and6wereleftempty(negativecontrol).Animalswerekilledonday14andthedefectsweredissectedandpreparedforhistologicalassessment.Quantitativeanalysisofnewboneformationwasmadeon100sections(50sectionsforeachgroup)usingimageanalysis.Results:Atotalof339%morenewbonewaspresentindefectsgraftedwithBio-Oss®Collagenthanthosegraftedwithcollagenmatrix(positivecontrol).Nobonewasformedinthenegativecontrolgroup.Conclusion:Bio-Oss®Collagenhastheeffectofstimulatingnewboneformationlocallycomparedwithcollagenmatrixinvivo.Bio-Oss®Collagenmaybeutilizedasabonegraftmaterial.Keywords:Bonegraft,Collagen,Bio-Oss®Collagen.INTRODUCTIONDiscoveryofbonesubstitutethatenhanceboneformationortoimprovebonehealingisneededforthetreatmentofbonetraumaorsurgery.Theuseofautogenousbonegraftsrequiresaharvestingprocedureatadonorsite,whichmeansincreasedmorbidity[1,2].Inaddition,graftresorptionisaproblem.ZinandWhitakershowedautogenousbonegrafthave65%ofbonelossaftergrafting[3].Onewaytoovercomethisproblemwouldbetousebonesubstitutesaloneasaosteoconductivescaffoldforboneregenerationfromtheresidualbone[4]orincombinationwithautogenousbone,whichalsohasosteoinductiveproperties[5].Earlystudieswithxenograftslikethe‘Kielbone’or‘Oswestrybone’halfacenturyagoshowedsideeffectslikesterilefistulasandtheirusewasdiscontinued[6-8].Bio-Oss®(GeistlischPharmaceutical,Wolhusen,Switzerland)isdeproteinizedbovinecancellousbonedevelopedinthelastdecadewithastructur...
