www.roche-applied-science.comForlifescienceresearchonly.Notforuseindiagnosticprocedures.X-tremeGENEsiRNATransfectionReagentForthetransfectionofsiRNAintoanimalcellsCat.No.044760930011mlCat.No.04476115001Multi-pack5�1ml(2000transfection)VersionJune2006Storeat�2to�8°C1.WhatthisProductDoesNumberofTransfectionExperimentsUsingstandardexperimentalconditions,onemilliliterofX-tremeGENEsiRNATransfectionReagenttransfectsHeLa,NIH3T3,Hek293cells,orothermammaliancellsinoverfourhundredwellsofa24-wellplate.Thisisequivalenttoover1,600transfectionsin96-wellplates.ContentsFormulationX-tremeGENEsiRNATransfectionReagentisapropri-etaryblendoflipidsandothercomponentssuppliedinaqueoussolu-tion,sterile-filtered,andpackagedinpolypropylenetubes.Thereagentisfreeofanycomponentsderivedfromanimals.StorageandStabilityX-tremeGENEsiRNATransfectionReagentisshippedatroomtem-peratureandisstabilizedforextendedstorageat+2to+8°Cthroughtheexpirationdateprintedonthelabelwhentightlyclosed.NAlwaysmixX-tremeGENEsiRNATransfectionReagentpriortouse(vortexforonesecondorinvert).Donotfreeze!AdditionalEquipmentandReagentsRequiredRefertothelistbelowforadditionalreagentsandequipmentrequiredtotransfectionexperiments.•Prewarmedsterile,serum-freeculturemediumwithoutadditivesorsupplements.Opti-MEM–1MediumisrecommendedfordilutionofthesiRNAandtheX-tremeGENEsiRNATransfectionreagent.•Mammaliancelllineofinterest•siRNAsolutionbetween0.2�g/�l(15pmoles/�l,i.e.15�M)and2�g/�l(150pmoles/�l,i.e.150�M)insterilebuffer.ApplicationX-tremeGENEsiRNATransfectionReagentisamulticomponentreagentthatformsacomplexwithsiRNA,andthendeliversitintoani-malcells.FeaturesofX-tremeGENEsiRNATransfectionReagentinclude:•Hightransfectionefficiencyinmanycommoncelltypes,includingHeLa,NIH3T3,Hek293,PC-3andCOS-7.•TheuseofonesinglereagentforsiRNA-andcotransfection-basedRNAexperiments.•Lowcytotoxicity,thechangeofmediaaftertheadditionoftransfec-tioncomplexisnotrequired.•Functionsexceptionallywellinthepresenceorabsenceofserum;eliminatesthenee...
