JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:GenerationofNeuralStemCellsfromDiscardedHumanFetalCorticalTissueJieLu1,LaurentC.Delli-Bovi2,JonathanHecht3,RebeccaFolkerth4,VolneyL.Sheen11DepartmentofNeurology,BethIsraelDeaconessMedicalCenter2DepartmentofObstetricsandGynecology,BrighamandWomen'sHospital3DepartmentofPathology,BethIsraelDeaconessMedicalCenter4DepartmentofPathology,DivisionofNeuropathology,BrighamandWomen'sHospitalCorrespondenceto:VolneyL.Sheenatvsheen@bidmc.harvard.eduURL:http://www.jove.com/video/2681DOI:doi:10.3791/2681MaterialsNameCompanyCatalogNumberCommentsKNOCKOUTDMEM/F12Invitrogen12660-012DissociationmediumStemProNSCSFMInvitrogenA10509-01CulturemediumFetalBovineSerumInvitrogen10091-148FrozenmediumHankssolution(-Ca2+,-Mg2+)Invitrogen14175-095DissociationmediumDMSOSigma-AldrichD2650FrozenmediumEDTASigma-Aldrich431788DissociationmediumParaformaldehydeSigma-Aldrich158127FixationsolutionbFGFR&DSystems234-FSEDifferentiationmediumSHHR&DSystems1845-SHDifferentiationmediumPDGF-AAR&DSystems221-AADifferentiationmediumB27Invitrogen17504-044DifferentiationmediumMouseAnti-MAP2Sigma-AldrichM23201:200RabbitAnti-DCXCellSignalingTechnology4604s1:200RabbitAnti-GFAPDakoZ03341:200RabbitAnti-S100BDakoZ03111:200RabbitAnti-O1giftsofProfessorTimothyVartanian*1:50RabbitAnti-O4GiftsofProfessorTimothyVartanian*1:5040μmcellstrainerBDBiosciences352340*TimothyVartanian,MD,PhD,DepartmentofNeurologyandNeuroscience,WeillCornellMedicalCollege,NewYork,USA
