JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsJune2011|52|e2954|Page1of11VideoArticleDevelopmentofCell-typespecificanti-HIVgp120aptamersforsiRNAdeliveryJiehuaZhou1,HaitangLi1,JaneZhang2,SwiderskiPiotr3,JohnRossi11DepartmentofMolecularandCellularBiology,BeckmanResearchInstituteofCityofHope2GraduateSchoolofBiologicalSciences,BeckmanResearchInstituteofCityofHope3SharedResource-DNA/RNAPeptide,BeckmanResearchInstituteofCityofHopeCorrespondenceto:JohnRossiatJrossi@coh.orgURL:http://www.jove.com/video/2954DOI:doi:10.3791/2954Keywords:Immunology,Issue52,SELEX(SystematicEvolutionofLigandsbyEXponentialenrichment),RNAaptamer,HIV-1gp120,RNAi(RNAinterference),siRNA(smallinterferingRNA),cell-typespecificdeliveryDatePublished:6/23/2011Citation:Zhou,J.,Li,H.,Zhang,J.,Piotr,S.,Rossi,J.DevelopmentofCell-typespecificanti-HIVgp120aptamersforsiRNAdelivery.J.Vis.Exp.(52),e2954,doi:10.3791/2954(2011).AbstractTheglobalepidemicofinfectionbyHIVhascreatedanurgentneedfornewclassesofantiretroviralagents.Thepotentabilityofsmallinterfering(si)RNAstoinhibittheexpressionofcomplementaryRNAtranscriptsisbeingexploitedasanewclassoftherapeuticsforavarietyofdiseasesincludingHIV.ManypreviousreportshaveshownthatnovelRNAi-basedanti-HIV/AIDStherapeuticstrategieshaveconsiderablepromise;however,akeyobstacletothesuccessfultherapeuticapplicationandclinicaltranslationofsiRNAsisefficientdelivery.Particularly,consideringthesafetyandefficacyofRNAi-basedtherapeutics,itishighlydesirabletodevelopatargetedintracellularsiRNAdeliveryapproachtospecificcellpopulationsortissues.TheHIV-1gp120protein,aglycoproteinenvelopeonthesurfaceofHIV-1,playsanimportantroleinviralentryintoCD4cells.Theinteractionofgp120andCD4thattriggersHIV-1entryandinitiatescellfusionhasbeenvalidatedasaclinicallyrelevantanti-viralstrategyfordrugdiscovery.Herein,wefirstlydiscusstheselectionandidentificationof2'-Fmodifiedanti-HIVgp120RNAaptamers.UsingaconventionalnitrocellulosefilterSELEXmethod,severalnewaptamerswi...
