JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:PouringandRunningaProteinGelbyreusingCommercialCassettesAlexanderC.Hwang1,ParisH.Grey1,KatrinaCuddy1,DavidG.Oppenheimer1,2,31DepartmentofBiology,UniversityofFlorida2UFGeneticsInstitute,UniversityofFlorida3PlantMolecular&CellularBiologyProgram,UniversityofFloridaCorrespondenceto:DavidG.Oppenheimeratoppenhe@ufl.eduURL:http://www.jove.com/video/3465DOI:doi:10.3791/3465MaterialsNameCompanyCatalogNumberCommentsInvitrogenNupageNovexMinigelCassette(1.0mm)InvitrogenNC2010Availablein2,5,10,12,15and2DwellsPlasticEpoxyLoctite108771ElectricalTapeScotchCo.125mLsidearmflaskPyrex53604XTris-Baseresolvinggelbuffer1.5MTris-basepH8.7acrylamideFisherScientific01065-500Bis-acrylamideMPBiomedicals80017330.8%acrylamidesolution30:0.8acrylamide:bis-acrylamideinIRG-H2O10%APSFisherScientificBP179-250.1gammoniumpersulfatein1mlIRG-H2O,preparedimmediatelybeforeuseTEMEDAcrosOrganics1384505001-butanolsaturatedwith1Xresolvinggelbuffer5:21-butanolto1Xresolvinggelbuffer4XTris-Basestackinggelbuffer1.5MTris-basepH6.8GelcombInvitrogenNC301515well,1.0mmthicknessImpulseSealerTEWElectricHeatingEquipmentHeatSealablePouchKapak/Scotchpak1pintsize(6.5"x8")Gelstoragebuffer1Xstackinggelbufferwith0.1%sodiumazideNovexSDSTris-Glycinesamplebuffer(2X)InvitrogenLC2676DTTFisherScientificBP172-510XTris-GylcineRunningbufferInvitrogenLC26751Lof10Xstock:29.0gTris-Base,144.0gGlycine,10.0gSDSXCellSureLockMini-CellInvitrogenEI0001GelKnifeInvitrogenEI9010SimplyBlueSafeStainInvitrogenLC6065
