JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:Bromodeoxyuridine(BrdU)LabelingandSubsequentFluorescenceActivatedCellSortingforCulture-independentIdentificationofDissolvedOrganicCarbon-degradingBacterioplanktonStevenRobbins*1,JishaJacob*1,XinxinLu1,MaryAnnMoran2,XiaozhenMou11BiologicalSciences,KentStateUniversity2MarineSciences,UniversityofGeorgia(UGA)*TheseauthorscontributedequallyCorrespondenceto:XiaozhenMouatxmou@kent.eduURL:http://www.jove.com/video/2855DOI:doi:10.3791/2855MaterialsNameTypeCompanyCatalogNumberCommentsBrdUReagentSigma-AldrichB5002-5GLysozymeReagentSigma-AldrichL6876-5GProteinaseKReagentSigma-AldrichP2308-25MGInSituCellProliferationKit,FLUOSKitRocheGroup11810740001ConsumemoreofAnti-BrdU-FLUOSandIncubationbufferperreactionthansuggestedbythemanufacturer.Frame-SealIncubationChambersMaterialBio-RadSLF-1201PolycarbonateMembraneFilters(142-mm-diameter,1.0μm-pore-size)MaterialEMDMilliporeFALP14250PolycarbonateMembraneFilters(25-mm-diameter,0.2μm-pore-size)MaterialEMDMilliporeFGLP02500illustraPuReTaqReady-To-GoPCRBeadsKitGEHealthcare27-9559-01QIAquickgelextractionkitKitQiagen28704FailSafePCRSystemKitEpicentreBiotechnologiesFS99060
