JournalofVisualizedExperimentswww.jove.comCopyright©2010CreativeCommonsAttribution-NonCommercialLicensePage1of2MaterialsListfor:Genome-wideAnalysisusingChIPtoIdentifyIsoform-specificGeneTargetsMichaelL.Beshiri1,AbulIslam2,DannielleC.DeWaal1,WilliamF.Richter1,JenniferLove3,NuriaLopez-Bigas2,ElizavetaV.Benevolenskaya11DepartmentofBiochemistryandMolecularGenetics,UniversityofIllinoisChicago-UIC2ResearchUnitonBiomedicalInformatics,UniversitatPompeuFabra3GenomeTechnologyCore,WhiteheadInstituteforBiomedicalResearchCorrespondenceto:ElizavetaV.Benevolenskayaatevb@uic.eduURL:http://www.jove.com/video/2101DOI:doi:10.3791/2101MaterialsNameCompanyCatalogNumberComments37%formaldehydesolutionFisherScientificF79500BSAUSBCorp.,Affymetrix10852chloroform/isoamylalcoholSigma-AldrichC0549DynabeadsProteinGInvitrogen100.04DEDTAFisherScientificBP120-500EGTASigma-AldrichE3889GenomicDNASamplePrepKitIlluminaFC-102-1001glycerolSigma-AldrichG5516glycogenBoehringerIngeheim901393HepesInvitrogen15630080KOHFisherScientificP250-500LiClSigma-AldrichL9650LowmolecularweightDNAladderNewEnglandBiolabsN3233SsodiumdeoxycholateSigma-AldrichD6750N-lauroylsarcosineMPBiomedicals194008NP-40Sigma-AldrichI3021PBSFisherScientificmt21040cvphenolSigma-AldrichP-4557PhusionDNAPolymeraseNewEnglandBiolabsF-540SproteinaseKGIBCO,byLifeTechnologies25530-049QIAquickPCRpurificationKitQiagen28104MinElutePCRpurificationKitQiagen28004QIAquickGelExtractionKitQiagen28704RNAseASigma-AldrichR4642SDSInvitrogen24730020TE(endofreeformaxi-prepkit)Qiagen19048TrisSigma-Aldrich154563TritonX-100FisherScientificBP151100UltraAgarose,Certifiedlow-rangeBio-Rad161-3106Oligonucleotidesequences2006Illumina,Inc.Allrightsreserved.•Sol_PCR_1Sequence5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’•Sol_PCR_2JournalofVisualizedExperimentswww.jove.comCopyright©2010CreativeCommonsAttribution-NonCommercialLicensePage2of2Sequence5’-CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-3’
