JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:TransFLP—AMethodtoGeneticallyModifyVibriocholeraeBasedonNaturalTransformationandFLP-recombinationMelanieBlokesch11GlobalHealthInstitute,SchoolofLifeSciences,EcolePolytechniqueFédéraledeLausanne(EPFL)Correspondenceto:MelanieBlokeschatmelanie.blokesch@epfl.chURL:http://www.jove.com/video/3761DOI:doi:10.3791/3761MaterialsNameCompanyCatalogNumberCommentsChitinflakesSigmaC9213AutoclavingrequiredOPTIONAL:Micropulser(incaseantibioticcassetteshouldbeexcisedbyFlprecombinaseencodedonpBR-flp)Biorad165-2100Orcomparableelectroporators
