王润堃,陆汉强,黄秋生.口咽鳞状细胞癌中HPVDNA检测的研究进展[J].中南医学科学杂志,2023,51(5):793-796.[收稿日期]2022-12-13[修回日期]2023-04-20[基金项目]江苏省卫生健康委员会(22020056)[作者简介]王润堃,硕士研究生,研究方向为头颈部鳞状细胞癌,E-mail为wa498192046@qq.com。陆汉强,博士,副主任医师,硕士研究生导师,研究方向为头颈部鳞状细胞癌,E-mail为luhanqiang@163.com。通信作者黄秋生,主任医师,硕士研究生导师,研究方向为头颈部鳞状细胞癌,E-mail为shengqiuhuang@163.com。DOI:10.15972/j.cnki.43-1509/r.2023.05.044·文献综述·口咽鳞状细胞癌中HPVDNA检测的研究进展王润堃,陆汉强,黄秋生江苏大学附属医院耳鼻咽喉-头颈外科,江苏镇江212000[摘要]随着世界范围对烟草、酒精危害认识的增加,烟草、酒精等高危因素引起的口咽鳞状细胞癌(OPSCC)的发病率在世界范围内有所下降,但人乳头瘤病毒(HPV)相关性OPSCC的发病率在逐渐上升,其造成的疾病负担也日益加重。目前已经开发了几种用于检测OPSCC中HPVDNA的方法,每种方法在灵敏度、特异度和操作难度上有着各自的优缺点。本文总结了目前具有前景的检测OPSCC中HPVDNA的方法,并对目前的研究进展进行综述。[关键词]人乳头瘤病毒;口咽鳞状细胞癌;环介导等温扩增技术;数字液滴PCR;第二代测序[中图分类号]R739.8[文献标识码]AResearchprogressofHPVdetectioninoropharyngealcancerWANGRunkun,LUHanqiang,HUANGQiushengDepartmentofOtorhinolaryngology-HeadandNeckSurgery,AffiliatedHospitalofJiangsuUniversity,Zhenjiang212000,Jiangsu,China[ABSTRACT]Withtheincreasingawarenessoftheharmoftobaccoandalcoholworldwide,theincidenceofsquamouscellcarcinomaoftheoropharynx(OPSCC)causedbyhighriskfactorssuchastobaccoandalcoholhasdecreasedworld-wide.However,theincidenceofhumanpapillomavirus(HPV)-relatedOPSCCisgraduallyincreasing,andthediseaseburdencausedbyHPVisalsoincreasing.SeveralmethodshavebeendevelopedtodetectHPVDNAinOPSCC,andthesemethodshavetheirownadvantagesanddisadvantagesintermsofsensitivity,specificity,andoperationaldifficulty.ThisarticlesummarizesthecurrentpromisingmethodsforthedetectionofHPVDNAinOPSCCandreviewsthecurrentre-searchprogress.[KEYWORDS]HPV;OPSCC;LAMP;ddPCR;NGS口咽鳞状细胞癌(squamouscellcarcinomaoftheoropharynx,OPSCC)是头...
