CircularRNAcSMARCA5inhibitsgrowthandmetastasisinhepatocellularcarcinomaGraphicalabstractAuthorsExon16Exon15DHX9↑I16RCI14RCExon17Exon14Exon16Exon15miR-17-3pmiR-181b-5pBacksplicing↓Pre-mRNAofSMARCA5cSMARCA5↓FreemiR-17-3p,miR-181b-5p↑TIMP3↓HCCgrowth,metastasis↑↑Upregulated↓DownregulatedIntronComplementaryHighlights�CircularRNAsinhumanHCCwereidentifiedusingRNA-sequencing.�CircularRNAcSMARCA5wasdownregulatedinHCCandassociatedwithpoorprognosis.�DownregulationofcSMARCA5inHCCwasattributedtotheupregulationofDHX9.�cSMARCA5inhibitedHCCgrowthandmetastasisbothinvitroandinvivo.�cSMARCA5actedasthespongeofmiR-17-3pandmiR-181b-5ptoupregulateTIMP3.JianYu,Qing-guoXu,Zhen-guangWang,...,Shu-hanSun,FuYang,Wei-pingZhouCorrespondenceyangfusq1997@smmu.edu.cn(F.Yang),ehphwp3@126.com(W.-p.Zhou)LaysummaryHerein,westudiedtheroleofcSMARCA5,acircularRNA,inhepatocellularcarci-noma.OurinvitroandinvivodatashowedthatcSMARCA5inhibitsthegrowthandmigrationofhepatocellularcarcinomacells,makingitapotentialtherapeutictarget.http://dx.doi.org/10.1016/j.jhep.2018.01.012�2018EuropeanAssociationfortheStudyoftheLiver.PublishedbyElsevierB.V.Allrightsreserved.J.Hepatol.2018,68,1214–1227ResearchArticleMolecularandCellBiologyJOURNALOFHEPATOLOGYCircularRNAcSMARCA5inhibitsgrowthandmetastasisinhepatocellularcarcinomaJianYu1,y,Qing-guoXu1,y,Zhen-guangWang1,y,YuanYang1,LingZhang2,Jin-zhaoMa2,Shu-hanSun2,FuYang2,⇑,Wei-pingZhou1,⇑1TheThirdDepartmentofHepaticSurgery,EasternHepatobiliarySurgeryHospital,SecondMilitaryMedicalUniversity,Shanghai,China;2TheDepartmentofMedicalGenetics,SecondMilitaryMedicalUniversity,Shanghai,ChinaBackground&Aims:Inrecentyears,circularRNAs(circRNAs)havebeenshowntohavecriticalregulatoryrolesincancerbiol-ogy.However,thecontributionsofcircRNAstohepatocellularcarcinoma(HCC)remainlargelyunknown.Methods:cSMARCA5(acircRNAderivedfromexons15and16oftheSMARCA5gene,hsa_circ_0001445)wasidentifiedbyRNA-sequencingandvalidatedbyquantitativereversetranscriptionPCR.TheroleofcSMARCA5inHCCprogres...
