ResourceLandscapeofInfiltratingTCellsinLiverCancerRevealedbySingle-CellSequencingGraphicalAbstractHighlightsdSingle-cellRNA-seqrevealsdistinctivefunctionalcompositionofTcellsinHCCdClonalenrichmentofinfiltratingTregsandexhaustedCD8TcellsinHCCdLAYNislinkedtothesuppressivefunctionoftumorTregandexhaustedCD8TcellsdCombinedexpressionandTCR-basedanalysesshowconnectivityamongTcellsubsetsAuthorsChunhongZheng,LiangtaoZheng,Jae-KwangYoo,...,WenjunOuyang,JirunPeng,ZeminZhangCorrespondencewouyang@amgen.com(W.O.),pengjr@medmail.com.cn(J.P.),zemin@pku.edu.cn(Z.Z.)InBriefAnalysisofTcellpopulationsfromhepatocellularcarcinomapatientsusingsingle-cellsequencingrevealsdistinctsubtypesandclonalexpansionofinfiltratinglymphocytes.Zhengetal.,2017,Cell169,1342–1356June15,2017ª2017ElsevierInc.http://dx.doi.org/10.1016/j.cell.2017.05.035ResourceLandscapeofInfiltratingTCellsinLiverCancerRevealedbySingle-CellSequencingChunhongZheng,1,7LiangtaoZheng,2,7Jae-KwangYoo,3,7HuahuGuo,4,5,6,7YuanyuanZhang,1,7XinyiGuo,1,7BoxiKang,1RuozhenHu,3JulieY.Huang,3QimingZhang,1ZhouzeruiLiu,1MinghuiDong,1XuedaHu,1WenjunOuyang,3,*JirunPeng,4,5,6,*andZeminZhang1,2,8,*1BIOPIC,BeijingAdvancedInnovationCenterforGenomics,andSchoolofLifeSciences,PekingUniversity,Beijing100871,China2Peking-TsinghuaCenterforLifeSciences,AcademyforAdvancedInterdisciplinaryStudies,PekingUniversity,Beijing100871,China3DepartmentofInflammationandOncology,AmgenInc.,SouthSanFrancisco,CA94080,USA4DepartmentofSurgery,BeijingShijitanHospital,CapitalMedicalUniversity,Beijing100038,China5NinthSchoolofClinicalMedicine,PekingUniversity,Beijing100038,China6SchoolofOncology,CapitalMedicalUniversity,Beijing100038,China7Theseauthorscontributedequally8LeadContact*Correspondence:wouyang@amgen.com(W.O.),pengjr@medmail.com.cn(J.P.),zemin@pku.edu.cn(Z.Z.)http://dx.doi.org/10.1016/j.cell.2017.05.035SUMMARYSystematicinterrogationoftumor-infiltratinglym-phocytesiskeytothedevelopmentofimmunother-apiesandthepredictionoftheirclinicalresponsesincancers.Here,weperf...
