ceRNA-The Long Noncoding RNA TTTY15, Which Is Located on the Y Chromosome, Promotes Prostate Cancer Progression by Sponging let-7.pdfVIP免费

PlatinumPriority–ProstateCancerEditorialbyXXXonpp.x–yofthisissueTheLongNoncodingRNATTTY15,WhichIsLocatedontheYChromosome,PromotesProstateCancerProgressionbySponginglet-7Guang’anXiaoa,b,y,JingjingYaoa,b,y,DepeiKonga,b,y,ChenYea,b,RuiChena,b,LiLic,TaoZengc,LiujunWangc,WeiZhanga,b,XiaoleiShia,b,TieZhoua,b,JingLia,b,YueWangb,c,ChuanLiangXua,b,JunfengJianga,b,c,**,YinghaoSuna,b,*aDepartmentofUrology,ShanghaiChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai,China;bShanghaiKeyLaboratoryofCellEngineering,SecondMilitaryMedicalUniversity,Shanghai,China;cDepartmentofHistologyandEmbryology,CollegeofBasicMedicine,SecondMilitaryMedicalUniversity,Shanghai,ChinaEUROPEANUROLOGYXXX(2018)XXX–XXXavailableatwww.sciencedirect.comjournalhomepage:www.europeanurology.comArticleinfoArticlehistory:AcceptedNovember6,2018AssociateEditor:JamesCattoStatisticalEditor:AndrewVickersKeywords:CRISPR/Cas9LongnoncodingRNAsProstatecancerTTTY15YchromosomeAbstractBackground:Thelinkbetweenprostatecancer(PCa)developmentandaberrantex-pressionofgeneslocatedontheYchromosomeremainsunclear.Objective:ToidentifyY-chromosomallongnoncodingRNAs(lncRNAs)withcriticalrolesinPCaandtoclarifythecorrespondingmechanisms.Design,setting,andparticipants:AberrantlyexpressedlncRNAsontheYchromosomewereidentifiedusingtranscriptomeanalysisofPCaclinicalsamplesandcelllines.BiologicalfunctionsandmolecularmechanismsofthelncRNAswererevealedusinginvitroandinvivoexperimentalmethods.Outcomemeasurementsandstatisticalanalysis:Experimentsandoutcomemeasure-mentswereperformedinduplicateortriplicate.Wilcoxonsigned-ranktestwasemployedforcomparisonofRNAlevelsinclinicalcohorts.Analysisofvariancewasemployedforcomparisonsamongmultiplegroups.Resultsandlimitations:InmostpatientswithPCa,TTTY15wasthemostelevatedlncRNAlocatedontheYchromosome.KnockoutofthislncRNAbytwodifferentCRISPR-Cas9strategiessuppressedPCacellgrowthbothinvitroandinvivo.TTTY15promotedPCabyspongingthemicroRNAlet-7,consequentlyincreasingCDK6andFN1expression.FOXA1isanupstreamr...