ArticleTranslationofCircRNAsGraphicalAbstractHighlightsdSpecificcircRNAsareassociatedwithtranslatingribosomesdRibosomefootprintingreadsmatchterminationcodonsignatureforcircMbldcircMbl3-derivedproteinisdetectedbymassspectrometrydcircRNAsaretranslatedinvitroandinvivoinacap-independentmannerAuthorsNagarjunaReddyPamudurti,OsnatBartok,MarvinJens,...,MarinaChekulaeva,NikolausRajewsky,SebastianKadenerCorrespondenceskadener@mail.huji.ac.ilInBriefPamudurtietal.showthatasubsetofcircRNAsistranslated.ThesecircRNAsgenerallysharethestartcodonwiththehostingRNA,encodeproteinswithspecificproteindomains,andaretranslatedinacap-independentmanner.Pamudurtietal.,2017,MolecularCell66,1–13April6,2017ª2017TheAuthor(s).PublishedbyElsevierInc.http://dx.doi.org/10.1016/j.molcel.2017.02.021MolecularCellArticleTranslationofCircRNAsNagarjunaReddyPamudurti,1,6OsnatBartok,1,6MarvinJens,2,6ReutAshwal-Fluss,1,6ChristinStottmeister,2LarissaRuhe,3MorHanan,1EmanuelWyler,4DanielPerez-Hernandez,5EvelynRamberger,5ShlomoShenzis,1MosheSamson,1GunnarDittmar,5MarkusLandthaler,4MarinaChekulaeva,3NikolausRajewsky,2andSebastianKadener1,7,*1BiologicalChemistryDepartment,SilbermanInstituteofLifeSciences,TheHebrewUniversityofJerusalem,Jerusalem91904,Israel2SystemsBiologyofGeneRegulatoryElements,Max-Delbr€uck-CenterforMolecularMedicine,Berlin13125,Germany3NonCodingRNAsandMechanismsofCytoplasmicGeneRegulation,Max-Delbr€uck-CenterforMolecularMedicine,Berlin13125,Germany4RNABiologyandPosttranscriptionalRegulation,Max-Delbr€uck-CenterforMolecularMedicine,Berlin13125,Germany5MassSpectrometryCoreUnit,Max-Delbr€uck-CenterforMolecularMedicine,Berlin13125,Germany6Co-firstauthor7LeadContact*Correspondence:skadener@mail.huji.ac.ilhttp://dx.doi.org/10.1016/j.molcel.2017.02.021SUMMARYCircularRNAs(circRNAs)areabundantandevolu-tionarilyconservedRNAsoflargelyunknownfunc-tion.Here,weshowthatasubsetofcircRNAsistranslatedinvivo.Byperformingribosomefootprint-ingfromflyheads,wedemonstratethatagroupofcircRNAsisassociatedwithtrans...
