ArticleUVIrradiationInducesaNon-codingRNAthatFunctionallyOpposestheProteinEncodedbytheSameGeneGraphicalAbstractHighlightsdUVelicitselongationslowdownandrestrictstranscriptiontothe50endofgenesdUVinducesaswitchfromlongtoshortalternativelastexon(ALE)transcriptisoformsdASCC3shortandlongALEisoformshaveantagonisticfunctionsintheUVresponsedTheUV-inducedASCC3shortisoformfunctionsasalongnon-codingRNAAuthorsLauraWilliamson,MarcoSaponaro,StefanBoeing,...,MichaelHowell,AengusStewart,JesperQ.SvejstrupCorrespondencejesper.svejstrup@crick.ac.ukInBriefUVdamagegeneratesafunctionalnon-codingRNAthroughalternativepre-mRNAprocessingofadamageresponsefactortranscript,identifyingapathwayforrepurposingproteincodinggenesunderselectiveconditions.DataResourcesGSE91012Williamsonetal.,2017,Cell168,843–855February23,2017ª2017TheAuthors.PublishedbyElsevierInc.http://dx.doi.org/10.1016/j.cell.2017.01.019ArticleUVIrradiationInducesaNon-codingRNAthatFunctionallyOpposestheProteinEncodedbytheSameGeneLauraWilliamson,1MarcoSaponaro,1,2StefanBoeing,1,3PhilipEast,3RichardMitter,3TheodorosKantidakis,1GavinP.Kelly,3AnnaLobley,3JaneWalker,1BradleySpencer-Dene,4MichaelHowell,5AengusStewart,3andJesperQ.Svejstrup1,6,*1MechanismsofTranscriptionLaboratory,TheFrancisCrickInstitute,ClareHallLaboratories,SouthMimmsEN63LD,UK2InstituteofCancerandGenomicSciences,UniversityofBirmingham,VincentDrive,Edgbaston,BirminghamB152TT,UK3BioinformaticsandBiostatistics4ExperimentalHistopathology5HighThroughputScreeningLaboratoryTheFrancisCrickInstitute,1MidlandRoad,LondonNW11AT,UK6LeadContact*Correspondence:jesper.svejstrup@crick.ac.ukhttp://dx.doi.org/10.1016/j.cell.2017.01.019SUMMARYThetranscription-relatedDNAdamageresponsewasanalyzedonagenome-widescalewithgreatspatialandtemporalresolution.UponUVirradiation,aslow-downoftranscriptelongationandrestrictionofgeneactivitytothepromoter-proximal�25kbisobserved.ThisisassociatedwithashiftfromexpressionoflongmRNAstoshorterisoforms,incorporatingalternativelastexons(ALEs)thataremorepro...
