中国组织工程研究与临床康复第13卷第36期2009–09–03出版JournalofClinicalRehabilitativeTissueEngineeringResearchSeptember3,2009Vol.13,No.36ISSN1673-8225CN21-1539/RCODEN:ZLKHAH7073DepartmentofUrology,UnionHospitalAffiliatedtoFujianMedicalUniversity,Fuzhou350001,FujianProvince,ChinaCaiPeng★,Studyingformaster’sdegree,Physician,DepartmentofUrology,UnionHospitalAffiliatedtoFujianMedicalUniversity,Fuzhou350001,FujianProvince,Chinawarriormd@qq.comCorrespondenceto:ZhuShao-xing,Doctor,Chiefphysician,Associateprofessor,Master’ssupervisor,DepartmentofUrology,UnionHospitalAffiliatedtoFujianMedicalUniversity,Fuzhou350001,FujianProvince,Chinazsxing2005@126.comSupportedby:theNaturalScienceFoundationofFujianProvince,No.C0710018*Received:2009-06-12Accepted:2009-07-25全骨髓贴壁法分离培养大鼠骨髓间充质干细胞及其诱导分化*★蔡鹏，朱绍兴，苏一鸣，黄世勇Isolation,cultureandmulti-directionaldifferentiationofratbonemarrowmesenchymalstemcellsbyusingthewholebonemarrowadherencemethodCaiPeng,ZhuShao-xing,SuYi-ming,HuangShi-yongAbstractBACKGROUND:Thereisfewmesenchymalstemcells(MSCs)inthebonemarrow,aboutoneBMSCin1×104-1×105monocytes.Followinginvitroisolation,purificationandamplification,itissatisfactoryforinvivorequirement.OBJECTIVE:Toobservebiologicalcharacteristicsandpotentialityofmulti-directionaldifferentiationofBMSCsinvitro.DESIGN,TIMEANDSETTING:ThecytologicalinvitrostudywasperformedattheInstituteofUrinarySurgery,UnionHospitalAffiliatedtoFujianMedicalUniversityfromOctober2007toDecember2008.MATERIALS:Atotalof30cleanmaleSpragueDawleyratsofinbreedinglineweresuppliedbySilaike,Shanghai,China.METHODS:BMSCsfromratswereisolatedandculturedinvitrobyusingthewholebonemarrowadherencemethod.When80%-90%confluency,BMSCsweredigestedbytrypsin.Atthethirdpassage,BMSCswereinducedtodifferentiateintoosteoblastsandadipocytes.MAINOUTCOMEMEASURES:Cellmorphologywasobservedundertheinvertedphasecontrastmicroscope.BMSCssurfacemarkersweredetectedusingflowcytometry.Osteogenicabilitywasexaminedbyalizarinredstaining.Ad...
