2004;128:472-479JThoracCardiovascSurgUchimura,GuopingChen,MasayukiHara,JunMiyakeandHikaruMatsudaShigemitsuIwai,YoshikiSawa,HajimeIchikawa,SatoshiTaketani,EiichirocardiovascularprosthesisBiodegradablepolymerwithcollagenmicrospongeservesasanewbioengineeredhttp://jtcs.ctsnetjournals.org/cgi/content/full/128/3/472locatedontheWorldWideWebat:Theonlineversionofthisarticle,alongwithupdatedinformationandservices,is2004AmericanAssociationforThoracicSurgeryAssociationforThoracicSurgeryandtheWesternThoracicSurgicalAssociation.Copyright©istheofficialpublicationoftheAmericanTheJournalofThoracicandCardiovascularSurgeryonJune14,2009jtcs.ctsnetjournals.orgDownloadedfromBiodegradablepolymerwithcollagenmicrospongeservesasanewbioengineeredcardiovascularprosthesisShigemitsuIwai,MDaYoshikiSawa,MDaHajimeIchikawa,MDaSatoshiTaketani,MDaEiichiroUchimura,PhDbGuopingChen,PhDbMasayukiHara,PhDcJunMiyake,PhDbHikaruMatsuda,MDaObjective:Biodegradablematerialswithautologouscellseedinghaveattractedmuchinterestaspotentialcardiovasculargrafts.However,pretreatmentofthesematerialsrequiresacomplicatedandinvasiveprocedurethatcarriestheriskofinfection.Toavoidtheseproblems,wesoughttodevelopabiodegradablegraftmaterialcontainingcollagenmicrospongethatwouldpermittheregenerationofautologousvesseltissue.Theabilityofthismaterialtoaccelerateinsitucellular-izationwithautologousendothelialandsmoothmusclecellswastestedwithandwithoutprecellularization.Methods:Poly(lactic-co-glycolicacid)asabiodegradablescaffoldwascompoundedwithcollagenmicrospongetoformavascularpatchmaterial.Thesepoly(lactic-co-glycolicacid)–collagenpatcheswith(n�10)orwithout(n�10)autologousvesselcellularizationwereusedtopatchthecaninepulmonaryarterytrunk.Histologicandbiochemicalassessmentswereperformed2and6monthsaftertheimplantation.Results:Therewasnothrombusformationineithergroup,andthepoly(lactic-co-glycolicacid)scaffoldwasalmostcompletelyabsorbedinbothgroups.Histologicresultsshowedtheformationofanendothelialcellmonolayer,aparallelalignmentofs...
