医学分子生物学杂志,2006,3(6):423-425�JMedMolBio,l2006,3(6):423-425技术与方法资助项目:国家重点基础研究发展计划(973项目)(No�2005CB623905)作者简介:汪静,男,1978年生,硕士研究生通讯作者:陈晓明(电话:027-87651853,E-mai:lxmchenw@126�com)ThisworkwassupportedbytheSpecialFundsforMajorStateBasicPro-gramofChina(973Project)(No�2005CB623905)Author�sbriefintroduction:WANGJing,male,bornin1978,Gradu-atestudentformasterdegreeCorrespondingauthor:CHENXiaoming(Te:l86-27-87651853,E-mai:lxmchenw@126�com)蛋白质电泳考马斯亮蓝G250染色方法改良汪静1,2,袁琳1,陈晓明11武汉理工大学生物材料与工程研究中心�武汉市,4300702武汉市江岸区妇幼保健院�武汉市,430017【摘要】�目的�建立一种快速,简易的SDS-PAGE染色方法。方法�用考马斯亮蓝G250和磷酸作为染色液,用水作为脱色液。并和考马斯亮蓝R250染色方法比较。结果�该方法染色背景浅,脱色简便,且灵敏度和考马斯亮蓝R250染色方法相当。结论�改进的考马斯亮蓝G250染色方法更加简便、经济、快速。适合于快速分析。【关键词】�电泳;染色方法;考马斯亮蓝G250【中图分类号】�Q530AnImprovedTechniqueforProteinStainingwithCBBG250WANGJing1,2,YUANLin1,CHENXiaoming11BiomedicalMaterialsandEngineeringCenter,WuhanUniversityofTechnology,Wuhan,430070,China2Jiang�anMaternalandChildHealthHospital,Wuhan430017,China【Abstract】�Objective�ToestablishaquickandeasymethodforSDS-PAGEproteinsta-ining�Method�TheCoomassieBrilliantBlueG250wasdissolvedintotheethanolandphosphate,thentheSDS-PAGEwasstainedwiththesolution�Severalhoursafterstainingoftheprotein,theSDS-PAGEwaswashedwithH2O�Theresultswerecomparedwithtraditionalmethod(CoomassieBrilliantBlueR250).Result�Thegelatinstainedwiththissolutionhadaclearbackgroundanddestainingwaseasyandequallysensitiveascomparedwithtraditionalmethod�Conclusion�Themodifiedmethodwassimple,fas,teconomicalandsuitableforquick-testing�【Keywords】�electrophoresis;stainingmethod;CoomassieBrilliantBlueG250��在SDS聚丙烯酰胺凝胶(SDS-PAGE)电泳中,常用到氨基黑染色、考马斯亮蓝染色和银染色等方法。其中考马斯亮蓝染色方法具有染色灵敏度较高,操作较简单、假阳性率低的特点,是实验室...
