www.roche-applied-science.comForlifescienceresearchonly.Notforuseindiagnosticprocedures.yVersion12Contentversion:April2011Easy-to-usehot-startreactionmixforPCRusingtheLightCycler®480SystemCat.No.04707516001Cat.No.048873520015×1ml(5×100reactions,20�leach)10×5ml(10×500reactions,20�leach)Storethekitat�15to�25°CNKeepLightCycler®480SYBRGreenIMasterawayfromlight!LightCycler®480SYBRGreenIMaster2www.roche-applied-science.comLightCycler®480SYBRGreenIMasteryVersion12TableofContents1.WhatthisProductDoes.................................................................................................4NumberofTests4KitContents4StorageandStability4AdditionalEquipmentandReagentsRequired5Application52.HowToUsethisProduct...............................................................................................62.1BeforeYouBegin6SampleMaterial6NegativeControl6Primers6MgCl262.2ExperimentalProtocol7LightCycler®480InstrumentProtocol7PreparationofthePCRMix92.3RelatedProcedures10PreventionofCarry-OverContamination10Two-stepRT-PCR103.Results.............................................................................................................................11QuantificationAnalysis11MeltingCurveAnalysis124.Troubleshooting............................................................................................................135.AdditionalInformationonthisProduct....................................................................15HowthisProductWorks15TestPrinciple16References17QualityControl176.SupplementaryInformation........................................................................................186.1Conventions18TextConventions18Symbols186.2ChangestoPreviousVersion186.3OrderingInformation196.4DisclaimerofLicense19NOTICETOPURCHASER196.5Trademarks19PROTOCOLPROTOCOLwww.roche-applied-science.com3LightCycler®480SYBRGreenIMasteryVersion121.WhatthisProductDoesNumberofTestsThekitisdesignedfor•500reactions(Cat.No.04707516001)•5,000reactions(Cat.No.04...
