StemPro®Accutase®CellDissociationReagentStemProAccutaseCellDissociationReagentisaready-to-usecelldetachmentsolutionofproteolyticandcollagenolyticenzymes.Usefulfortheroutinedetachmentofcellsfromstandardtissuecultureplasticwareandadhesioncoatedplasticware,includingGeltrex™ReducedGrowthFactorBasementMembraneMatrix,CELLStart™andpolymers.Accutaseperformsexceptionallywellindetachingcellsfortheanalysisofcellsurfacemarkers,virusgrowthassay,quiescenceassaysbyserumstarvation,transformationassaysbyoncogenetransfection,neuralcrestcellmigrationassays,cellproliferation,cellhaptotaxsis,tumorcellmigrationassaysroutinecellpassage,productionscale-up(bioreactor),andflowcytometry.CelllinestestedforAccutaseapplicationincludesfibroblasts,keratinocytes,vascularendothelialcells,hepatocytes,vascularsmoothmusclecells,hepatocyteprogenitors,primarychickembryoneuronalcells,bonemarrowstemcells,adherentCHOandBHKcells,macrophages,293cells,L929cells,immortalizedmousetesticulargermcells,3T3,Vero,COS,HeLa,NT2,MG63,M24andA375metastaticmelanoma,gliomasU251,D54,HT1080fibrosarcomacells,Sf9insectcells,humanembryonicstemcells,humanmesenchymalstemcellsandhumanneuralstemcells.Accutasedoesnotcontainmammalianorbacterialderivedproducts.DescriptionCat.No.SizeStemProAccutaseCellDissiciationReagentA11105-011x100mLIntendedUseForresearchuseonly.CAUTION:Notintendedforhumanoranimaldiagnosticortherapeuticuses.PrecautionsDonotstoreAccutaseatroomtemperature.Accutaseisstablewhenstoredat2to8°Cupto2years.ItisrecommendedtothawAccutaseatroomtemperatureor4°Covernight.Donotthawat37°C.StorageStoreat-5to-20°C,Protectfromlight.ShelfLife24monthsUse:Note:Thefollowingproceduresaredesignedtodissociatecellson60mmdish.Volumesshouldbeadjustedaccordinglyfordesiredvesselsize.GeneralDissociation:1.Aspiratethemediumandwashwith4mLofDPBS(w/ocalciumandmagnesium,Cat.No14190).2.AddAccutasetoculturedishorflaskusingasepticproceduresat2mLper60mmsurfacearea(10mLper75cm2surfacearea).3.Returncultureto37°Cincubatorandallowcellstodetach5to10m...
