UserManualRatFetalNeuralStemCellsCatalognos.N7744-100,N7744-200Rev.date:7April2009Manualpartno.A11229MAN0001642iiiiiTableofContentsContentsandStorage...........................................................................................ivAdditionalProducts..............................................................................................vIntroduction........................................................................................1RatFetalNeuralStemCells(NSCs)....................................................................1PhenotypeMarkerExpressionofRatFetalNSCs............................................3Methods...............................................................................................5HandlingRatFetalNSCs......................................................................................5MediaRequirements..............................................................................................6PreparingCompleteStemPro®NSCSFM..........................................................7PreparingMatrixforAdherentCellCulture.....................................................8ThawingandEstablishingCells........................................................................10SubculturingCells................................................................................................12SubculturingCells(AdherentCulture)............................................................13SubculturingCells(SuspensionCulture).........................................................14FreezingCells........................................................................................................15DifferentiatingRatFetalNSCs...........................................................................17CharacterizingPhenotypeofRatFetalNSCs..................................................18Troubleshooting...................................................................................................20Appendix...............
