Chih-chienMTTtest:Day1:Seedcellsontoa96-wellplate(96wellsplate,1x104cellsperwell)Day2:Spindowncells(1000rpm,5min),AspirateallmediumwithaPasteurpipet(witha10µl“tip”inthefront)↓Rinsewithnewmedium(100µl/well)by八爪pipette↓discardmediumbysuction↓add100µl(medium+MTT)sol.toeachwell(*preparing:【10mlmedium+1mlMTT】/plate)5mgMTTin1mlPBS↓backtoincubatornomorethan4hr.↓spindowncells(1000rpm,5min),discardsup.↓add100µl(isopropanol(異丙醇)+HCl)670µl12NHClin200mlisopropanol(final-0.04NHCl)oradd100µlDMSO↓standfor2hr.↓measureO.D.value(570nm)
