Long Noncoding RNAs 2nd(1).pptxVIP免费

LongNoncodingRNAs:Genomics,Functions,Methodologies,ModesofActionsWeiGuifeng,guifengwei@gmail.comInstituteofBiophysics,ChineseAcademyofSciences2Reads(FASTQ)FASTQCMappingTophat2,STAR,SpliceMap,……TranscriptomeAssemblyCufflinks,Scripture,Trinity,……NoveltranscriptsQuantificationCuffcompareCufflinks,CuffnormDifferentiallyExpressedGenesCuffdiff,edgeR,DEG,DESeq,……PipelineforRNA-seqanalysisBED,GeneBed,GenePred,GTF/GFFWig,Bigwig,BedGraphFasta/FASTQ,SAM/BAM,VCF/BCFDataFileFormathttp://genome.ucsc.edu/FAQ/FAQformat.htmlCiChuetal.MolCell2011ChIRP(CHART,RAP)-seqChromatinisolationbyRNApurificationcapturehybridizationofRNAtargetsRNAantisensepurificationPNASScienceMolecularCellChIRP(CHART,RAP)-seqHOTAIR(CiChuetal.MolCell2011)Xist(JesseM.Engreitzetal.Science2013,MatthewD.Simonetal.Nature2013)Paupar(KeithWVanceetal.EMBOJ2014)Dali(VladislavaChaleietal.eLife2014)NEAT1andMALAT1(JasonA.Westetal.MolCell2014)PCGEM1(LiuqingYangetal.Nature2013)ChIRP(CHART,RAP)-seqChIRP(CHART,RAP)-seqprotocolhttp://www.jove.com/video/3912/chromatin-isolation-by-rna-purification-chirpLongnoncodingRNAsmayfunctionasorganizingfactorsthatshapethecellnucleus.RinnandGuttman.Science2014GenomicLandscapeofNEAT1andMALAT1locilncRNACO1CO2CHARTenrichesthelncRNAsNEAT1andMALAT1.NEAT1andMALAT1localizetoActivelyTranscribedGenomicLociNEAT1andMALAT1localizetoActivelyTranscribedGenomicLociNEAT1andMALAT1localizetoGenomicSitesintrans.highabundanceactintranslocalizationinnucleus,specialwithchromatinChIRP(CHART,RAP)-seqWhichlncRNAsaresuitableforChIRP-seqanalysis?Thankyou