Thispresentationisprotectedbycopyrightlaw.AuthorshiphasonlyCellularTechnologyLtd.DonotpublishorcopythispresentationwithoutpermissionfromtheAuthor.Pleasecontactja@immunospot.comElevatingELISPOTtoanExactScienceDr.HongweiLiu(Prof.Dr.PaulV.Lehmann)CellularTechnologyLtd.CTL,CaseWesternReserveUniversityThispresentationisprotectedbycopyrightlaw.AuthorshiphasonlyCellularTechnologyLtd.DonotpublishorcopythispresentationwithoutpermissionfromtheAuthor.Pleasecontactja@immunospot.comELISPOT:fieldsofapplication•Visualizingthesecretoryproductsofindividualcells•–detectingrarecells.•Thetechniqueofchoiceinimmunemonitoring:•Basicimmunology•Infectiousdiseases•Autoimmunity•Tumorimmunity•Transplantation•Allergies•VaccinedevelopmentThispresentationisprotectedbycopyrightlaw.AuthorshiphasonlyCellularTechnologyLtd.DonotpublishorcopythispresentationwithoutpermissionfromtheAuthor.Pleasecontactja@immunospot.comAdvantagesofELISPOTvs.others•Sensitivity:(detectionlimit<1/1million,andless:severalhundredfoldmoresensitivethanELISA,BeadArray,Tetramer,ICS,mRNAetc).•Functionalassay:•Lowcost:Instrumentation,reagentsandlaborareverylowcostrelativetoflowbasedtechniques.•Highthroughput:Singleinvestigatorcantestdozensofsamplesforhundredsofreactivateseachinasingleexperiment.•Lesssample:•Easeofuse:CTLcantrainexpertsin4daysThispresentationisprotectedbycopyrightlaw.AuthorshiphasonlyCellularTechnologyLtd.DonotpublishorcopythispresentationwithoutpermissionfromtheAuthor.Pleasecontactja@immunospot.comKarulinandLehmann,J.Immunol.,2000,ICS(A&C)andELISPOT(B&D)havesimilarresultsfordetectingcytokine(co)expressioninsingleTcellsThispresentationisprotectedbycopyrightlaw.AuthorshiphasonlyCellularTechnologyLtd.DonotpublishorcopythispresentationwithoutpermissionfromtheAuthor.Pleasecontactja@immunospot.comMajorStrength:Sensitivity:ELISPOTpermitsaccuratefrequencymeasurements
