2012年第16卷第24期实用临床医药杂志JoumalofCliniealMedicineinPractice·11·麦冬多糖对2型糖尿病大鼠肾脏的保护作用陆小元(江苏省姜堰市中医院药剂科，江苏姜堰，225400)摘要：目的观察麦冬多糖对2型糖尿病大鼠肾脏的保护作用。方法采用链脲佐菌素(STZ)诱导2型糖尿病大鼠模型，给予不同剂量麦冬多糖(200、400、800Irlg／kg)治疗6周后，检测各组大鼠空腹血糖(FBG)、肾脏指数、血肌酐(Scr)、尿素氮(BUN)含量，比较各组大鼠肾组织中总抗氧化能力(T-AOC)及过氧化氢(H2O2)的含量，Westernbolt检测肾组织中p47、核因子-KB(NF-KB)蛋白表达。结果模型组大鼠FBG、肾脏指数、Scr、BUN含量明显升高，’肾脏T．AOC明显降低，肾组织p47ph、NF-KB的表达及H202含量显著升高。麦冬多糖能明显降低糖尿病大鼠FBG、肾脏指数、Scr及BUN水平，提高肾脏T．AOC，降低肾脏p47ph、NF—KB的表达及H202的含量。结论麦冬多糖对2型糖尿病大鼠肾脏具有一定的保护作用，其机制可能与其抗氧化、下调糖尿病大鼠肾脏p47ph、NF—KB的表达有关。关键词：麦冬多糖；糖尿病肾病；尼克酰胺腺嘌呤二核苷酸氧化酶；氧化应激中图分类号：R587．1文献标志码：A文章编号：1672—2353(2012)24—0011—04Effectofophiopogon-p0lysaccharideonprotectionofthekidneyintype2diabeticratsLUXiaoyuan(JiangyanHospitalofTraditionalChineseMedicine，Jiangyan，Jiangsu，225400)ABSTRACT：ObjectiveToobserveeffectofophiopogon—polysaccharideonprotectionofkidneyintype2diabeticrats．MethodsStreptozotocin·induceddiabeticratsweregivenophiopogon-polysac-charide(200，400，800mg／kg)for6weeks．Thechangesinbloodglucose(FBG)，kidneyweightindex，andserumcreatinine(Scr)andbloodureanitrogen(BUN)weredeterminedaftertreatment．Thetotalantioxidantcapacity(T—AOC)andhydrogenperoxide(H2O2)inrenaltissueweremeasuredandcomparedbetweengroups．Inaddition，theexpressionsofp47。，nuclearfactor—KB(NF-KB)inthekidneytissuesweredeterminedbywesternbolt．ResultsBG，kidneyweightindexandScrandBUNinthemodelgroupincreasedsignificantly，renalT—AOCsignificantlydecreased．ThecontentsofrenalH202andexpressionsofrenalp47，NF—KBincreasedsignificantly．Comparedwithmodelgroup，thelevelofBG，kidneyweightindexandScrandBUNinophiopogon—polysaccharidegroupsdecrea...
