胶原海绵与骨髓基质成骨细胞体外联合培养的实验研究成洪泉翦新春*许春娇(中南大学湘雅医院口腔颌面外科湖南长沙410008)[摘要]目的:探索胶原海绵作为骨组织工程支架材料的可行性。方法:将人骨髓基质成骨细胞以1@107mL的密度接种于胶原海绵上进行体外培养,用相差显微镜、光镜、扫描电镜观察细胞在胶原海绵上的生长、增殖情况。结果:体外复合培养1d后,成骨细胞即开始粘附于胶原海绵上,培养7d后,分布于胶原海绵上的细胞增殖并分泌ECM。结论:胶原海绵可作为成骨细胞体外复合培养的支架材料。[关键词]骨组织工程成骨细胞胶原海绵[中图分类号]R329.2[文献标识码]A[文章编号]1671)7651(2004)05)0498)03ExperimentalStudyofBoneMarrowStromalOsteoblastsCulturewithCollagenSpongeinVitro.CHENGHong-quan,JIANXin-chun,XUChun-jiao.DepartmemtofOralandMaxillofacialSurgery,XiangyahospitalCen-tralSouthUniversity,Changsha410008.[Abstract]Objective:Toinvestigatethefeasibilityofcollagenspongeasscaffoldmaterialinbonetissueengineering.Methods:Humanbonemarrowstromalosteoblastswhichhadbeendifferentiatedandproliferatedwereseededincollagenspongeinadensityof1@107/mL.Invitro,thecell-scaffoldcomplexwasobservedbyphasecontrastmicroscope,lightmicroscopeandelectronicscanningmicroscopeinordertoevaluatetheinteractionbetweencellsandcollagensponge.Re-sults:Bonemarrowstromalosteoblastsseededinthreedimensioncollagenspongewerelivinginafloatingcondition.onedayafterbeingcocultured,theosteoblastsadheredtocollagensponge.Sevendayslater,theosteoblastsdifferentiatedandproliferatedincollagespongenetwork.AfewextrocellularmatricewasobservedbySEMafter7days.Conclusions:Thecollagenspongeisagoodscaffoldforosteoblaststhreedimensioncultureinvitro.[Keywords]BonetissueengineeringOsteoblastCollagensponge骨组织工程的构建必须选择合适的材料来作为细胞生长的支架。目前骨组织工程支架材料主要有两类:一类是生物衍生材料,由生物组织通过一系列理化方法处理而制得,包括天然骨、胶原、珊瑚骨、壳聚糖等;另一类为人工合成材料,包括羟基磷灰石、钙磷陶瓷、生物活性玻璃、聚脂类等。生物衍生材料具有来源丰富、造价低及有天然的网状孔隙结构等优点,越来越受到人们的重视。胶原是骨组织的主要成分之一,它为组织钙化提供...
