中国烟草科学ChineseTobaccoScience2023,44(4):49-57DOI:10.13496/j.issn.1007-5119.2023.04.007广东韶关烟区烟草镰刀菌根腐病病原鉴定及致病性分析沈会芳1，邓海滨2，杨祁云1，张景欣1，曾涛2，蒲小明1，孙大元1，林壁润1*（1.广东省农业科学院植物保护研究所，广东省植物保护新技术重点实验室，广州510640；2.广东省烟草科学研究所，广东韶关512026）摘要：为明确广东韶关烟草镰刀菌根腐病菌种类及致病力，在珠玑、始兴、南雄和乌茎采集根腐病样本，用组织分离法分离病原菌，盆栽接种带菌麦粒测定其致病性，观察菌株形态，基于EF-1α和β-Tub基因序列构建发育树鉴定病原菌。结果表明，获得45株镰刀菌，通过形态特征和分子技术鉴定为茄病镰刀菌Fusariumsolani、共享镰刀菌F.commune、藤仓镰刀菌F.fujikuroi和尖孢镰刀菌F.oxysporum，分别占比42.22%，26.67%、20.00%和11.11%，对烟草均有致病性，强弱排列为F.commune≈F.fujikuroi＞F.oxysporum＞F.solani。本研究可为广东韶关地区烟草镰刀菌根腐病的防治提供依据。关键词：烟草；镰刀菌根腐病；鉴定；致病性中图分类号：S435.72文献标识码：A文章编号：1007-5119（2023）04-0049-09IdentificationandPathogenicityAnalysisofTobaccoFusariumRootRotPathogensinShaoguan,GuangdongProvinceSHENHuifang1,DENGHaibin2,YANGQiyun1,ZHANGJingxin1,ZENGTao2,PUXiaoming1,SUNDayuan1,LINBirun1*(1.InstituteofPlantProtection,GuangdongAcademyofAgriculturalSciences,GuangdongProvincialKeyLaboratoryofNewTechniqueforPlantprotection,Guangzhou510640,China;2.GuangdongResearchInstituteofTobaccoSciences,Shaoguan,Guangdong512026,China)Abstract:ToclaritythepathogensoftobaccoFusariumrootrotandtheirpathogenicity,samplesshowingrootrotsymptomsfromZhuji,Shixing,NanxiongandWujinginGuangdongwerecollected.Strainswereisolatedbytissueseparationandtheirpathogenicitytotobaccoseedlingswasdeterminedbyinoculationofwheatgrainswithfungiinpottedtobaccoplants.Thestrainswereidentifiedbasedonmorphologicalcharacteristics,combinedwiththephylogeneticanalysismethodusingpartialsequencesofEF-1αandβ-Tub.45Fusariumstrainswereisolated.Theresultsofmorphologicalandmolecularbiologicalidentificationshowedthat45strainsbelongedtodifferentspecies,i.e.,Fusariumsolani,F.com...
