天然产物研究与开发NatProdResDev2023,35:1775-1781光质对等鞭金藻的生长和生物活性产物合成的影响钟彩荣"，陈钦常",许涵玥"，曾子航，徐绍丝’，车丹丹2，方静平1，何勇锦1.2*1福建师范大学生命科学学院；福建师范大学工业微生物教育部工程研究中心，福州350117摘要：本实验探究白光（400~700nm）、红光（620~650nm）和绿光（500~580nm）对等鞭金藻（Isochrysissp.IS0-FJ)的生长和生物活性产物积累的协同影响,以期摸索适宜的光质同时提高其生物活性产物。实验结果表明，等鞭金藻细胞在白光条件下具有最大的生物量。在生物活性产物合成方面，与白光和红光相比,采用绿光培养的等鞭金藻细胞更有利于岩藻黄素、胞外多糖和多不饱和脂肪酸的联产合成。这些结果可表明，等鞭金藻的生长和活性产物合成是受不同光质的调节，为今后定向调控等鞭金藻生长和活性产物合成提供实践依据和理论指导。关键词：等鞭金藻；光质；岩藻黄素；胞外多糖；多不饱和脂肪酸中图分类号：Q935D0I:10.16333/j.1001-6880.2023.10.014EffectofdifferentlightqualitiesonthegrowthandbioactivecompoundsbiosynthesisofIsochrysissp.ISO-FJZHONGCai-rong",CHENQin-chang",XUHan-yue',ZENGZi-hang",XUShao-si,CHEDan-dan'*2,FANGJing-ping",HEYong-jin'.2*'CollegeofLifeSciences,FujianNormalUniversity;2EngineeringResearchCenterofIndustrialMicrobiology,MinistryofEducation,FujianNormalUniversity,Fuzhou350117,ChinaAbstract:Inordertoexploretheappropriatelightqualitytosimultaneouslyimprovemultiplebioactivecompoundsproductivi-ties,thesynergisticeffectsofwhitelight(400-700nm),redlight(620-650nm)andgreenlight(500-580nm)onthemi-croalgalgrowthandbioactivecompoundsbiosynthesisofIsochrysissp.ISO-FJwereinvestigated.TheresultsshowedthatIsoch-rysissp.ISO-FJgavethehighestbiomassinthewhitelight.Intermofthebioactivecompounds,thegreenlightwasmoreben-eficialtothecoproductionoffucoxanthin,exopolysaccharidesandpolyunsaturatedfattyacidsinmicroalgalcells,incompari-sontothewhiteorredlight.TheseresultstogethersuggestedthatthegrowthandbioactivecompoundsbiosynthesisofIsochry-sissp.wereregulatedbylightqualities,whichcouldofferpracticalbasisandtheoreticalguidanceforthedirectedregulationofmicroalgalgrowthandbioactivecompound...
