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TechnicalManualCheckMate™/Flexi®VectorMammalianTwo-HybridSystemINSTRUCTIONSFORUSEOFPRODUCTC9360.Revised4/09Part#TM283PromegaCorporation·2800WoodsHollowRoad·Madison,WI53711-5399USATollFreeinUSA800-356-9526·Phone608-274-4330·Fax608-277-2516·www.promega.comRevised4/09Part#TM283Page11.Description..........................................................................................................12.ProductComponentsandStorageConditions............................................43.GeneralConsiderations....................................................................................5A.pFN10A(ACT)Flexi®Vector.............................................................................6B.pFN11A(BIND)Flexi®Vector............................................................................8C.pGL4.31[luc2P/GAL4UAS/Hygro]Vector.....................................................104.Protocols............................................................................................................12A.CloningintotheFlexi®Vectors........................................................................12B.VectorPurification,TransfectionandExperimentalDesign.......................135.Troubleshooting...............................................................................................156.References.........................................................................................................167.RelatedProducts..............................................................................................188.Appendix...........................................................................................................19A.pFN10A(ACT)Flexi®VectorRestrictionSites..............................................19B.pFN11A(BIND)Flexi®VectorRestrictionSites............................................21C.pGL4.31[luc2P/GAL4UAS/Hygro]VectorRestrictionSites.......................23D.ControlVectorMaps..........................................................