生物秀论坛网上搜集，更多精彩内容请访问http://www.bbioo.com/bbs/index.php1国内重点实验室分子生物学实验方法汇总国内重点实验室分子生物学实验方法汇总国内重点实验室分子生物学实验方法汇总PCR.....................................................................................................................................................2RT-PCR................................................................................................................................................4琼脂糖核酸电泳.................................................................................................................................6胶回收纯化DNA...............................................................................................................................6大肠杆菌质粒DNA的提取（碱裂解法）......................................................................................7乙醇沉淀DNA...................................................................................................................................8酶切.................................................................................................................................................8连接...................................................................................................................................................8感受态细胞的制备.............................................................................................................................9转化.................................................................................................................................................10重组子的筛选和鉴定.......................................................................................................................10真核细胞的转染...............................................................................................................................11转染细胞的稳定筛选........................................................................
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