2017 protein function(1).pptVIP免费

Proteinfunctions郭占云教授2017-09-282Proteinfunctions（蛋白质的功能）Structuralproteins结构蛋白Activelyfunctionalproteins功能蛋白keratins,collagens,cytoskeletons,extracellularmatrix………enzymes,hormones,receptors,transporters,ionchannels,transcriptionfactors………3“permanentwaving”（烫发）silkfibroin丝心蛋白-keratin角蛋白4cytoskeleton细胞骨架actin微丝microtubule微管OpossumKidneyEpithelialCells(OKLine)http://www.olympusfluoview.com/gallery/cells/ok/okexlarge3.html56肌肉收缩(M)GLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQGmyoglobin肌红蛋白8Hemegroup血红素9Protein-ligandinteraction（蛋白质-配体之间的相互作用）P+LPLKa=[PL][P][L]Kd=[P][L][PL]Y=BindingsiteoccupiedTotalbindingsite[PL][PL]+[P]==[L][L]+KdKa,associationconstant;Kd,dissociationconstant.(withaconcentrationunit)10myoglobin+O2myoglobin·O2Y=[L][L]+Kd=[O2][O2]+[O2]0.5=pO2pO2+P5011Hemoglobin血红蛋白http://en.wikipedia.org/wiki/HemoglobinTwosubunitsandtwosubunits.(M)VLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR(M)VHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH12myoglobin13Hemoglobinαchain14myoglobin&hemoglobinαchain15myoglobinvs.hemoglobin?16myoglobin:O2storagehemoglobin:O2transportationMb:myoglobin(肌红蛋白);Hb:hemoglobin(血红蛋白).myoglobinvs.hemoglobinHbbinds2,3-biphosphoglycerate(2,3-二磷酸甘油）inredbloodcells17Y=[pO2]n[pO2]n+P50nn=1,hyperboliccurve（双曲线）;Singleorindependentbinding;n>1,sigmoidalcurve（S型曲线）;cooperativebinding.Hb+nO2Hb·(O2)nHillcoefficient,n,reflectsthedegreeofcooperativity（协同性）.18http://en.wikipedia.org/wiki/HemoglobinHemoglobinisanallostericprotein（别构蛋白）.19SomethingelsetoknowabouthemoglobinHemoglobinbindsCOwithhigheraffinity（亲和力）;Sickle-cellanemia（镰刀形贫血症）iscausedbyhemoglobinmutationE6V.20ATPsynthase:ahighlycomplexmolecularmachine