2017_enzyme_3(1).pptVIP免费

1Enzymes2017-10-24chunguangwang@tongji.edu.cn王春光2]S[]S[mmaxKVvMichaelis-Mentenequationderivedfromasimplifiedsimplesituation3Regulationsofenzymeactivityreversibleregulations可逆调控zymogenactivationirreversibleregulations不可逆调控reversibleinhibitorsallostericmodulatorsirreversibleinhibitorspost-translationalmodifications……4S+EESP+E1k1k2k+IEIKIproductiveunproductiveCompetitiveinhibitor竞争性抑制剂5Competitiveinhibitor竞争性抑制剂Immax[I]1α,]S[α]S[KKVv6Competitiveinhibitor竞争性抑制剂Immax[I]1α,]S[α]S[KKVv7E+SESP+E1k1k2k+IESIKInoncompetitiveinhibitor非竞争性抑制剂+IEI+SKI8noncompetitiveinhibitor非竞争性抑制剂Immax[I]1α,]S[α]S[KKVv9S+EESP+E1k1k2k+IESIKIproductiveunproductiveuncompetitiveinhibitor反竞争性抑制剂10uncompetitiveinhibitor反竞争性抑制剂Immax[I]1α,]S[α]S[KKVv11Regulationofenzymeactivityreversibleregulationzymogenactivationirreversibleregulationreversibleinhibitorsallostericmodulatorsirreversibleinhibitorspost-translationalmodifications……12Allostericenzymesundergoconformationalchanges13Proteinphosphorylationphosphorylasephosphatase激酶磷酸化酶磷酸酶kinasephosphorylysishydrolysiskinasephosphorylasephosphatasekinasephosphorylasephosphatase17Cascadeamplification级联放大18TheNobelPrizeinPhysiologyorMedicine1992“fortheirdiscoveriesconcerningreversibleproteinphosphorylationasabiologicalregulatorymechanism”EdmondH.FischerUniversityofWashingtonSeattle,WA,USAb.1920(inShanghai)EdwinG.KrebsUniversityofWashingtonSeattle,WA,USAb.191819Irreversibleinhibitor20Activationofzymogenbyproteolyticcleavage21绝大多数酶都是蛋白质。绝大多数生物学功能都由蛋白质完成。