第9卷第3期2023年6月生物化工BiologicalChemicalEngineeringVol.9No.3Jun.2023文章编号：2096-0387（2023）03-05异槲皮苷-β-环糊精包合物的制备及溶解度研究朱伶俐1*，周委2（1.武汉软件工程职业学院，湖北武汉430205；2.武汉市药物增溶工程技术研究中心，湖北武汉430205）摘要：采用共沉淀法制备了异槲皮苷-β-环糊精固体包合物，用紫外、红外光谱进行包合物表征，同时利用紫外分光光度法建立了包合物中异槲皮苷的含量测定方法，并考察了方法的精密度和回收率。结果表明，异槲皮苷的日内和日间精密度均小于2%，平均回收率为96.04%～99.88%。在此基础上，对异槲皮苷在β-环糊精溶液中的相溶解度进行研究，发现异槲皮苷与β-环糊精形成了1∶1型包合物；相溶解度结果显示异槲皮苷被制备成固体包合物后，在本实验浓度范围内溶解度最大可提高为包合前的7.31倍。关键词：异槲皮苷；β-环糊精；包合物；溶解度中图分类号：O657.32文献标识码：APreparationTechnologyandSolubilityofIsoquercitrin-β-CyclodextrinInclusionComplexZHULingli1*,ZHOUWei2(1.WuhanVocationalCollegeofsoftwareofEngineering,Wuhan430205,China;2.WuhanDrugSolubilizationandDeliveryTechnologyResearchCenter,Wuhan430205,China)Abstract:Theisoquercitrin-β-cyclodextrinsolidinclusioncomplexispreparedbyco-precipitationmethod,andtheinclusioncomplexischaracterizedbyultravioletandinfraredspectroscopy.Thedeterminationmethodofisoquercitrinininclusioncompoundisestablishedbyultravioletspectrophotometry,andtheprecisionandrecoveryrateareinvestigated.Theresultsshowthattheintra-dayandinter-dayprecisionofisoquercitrinarebothlessthan2%,andtheaveragerecoveryrateis96.04%～99.88%,respectively.Onthisbasis,thephasesolubilityofisoquercitrininβ-cyclodextrinsolutionisstudied,anditisfoundthatisoquercitrinandβ-cyclodextrinformeda1∶1inclusioncomplex.Thephasesolubilitystudyshowsthatafterthepreparationofisoquercetinasasolidinclusioncomplex,themaximumincreasewithintheconcentrationrangeofthisexperimentis7.31timesthatofbeforeinclusion.Keywords:isoquercitrin;β-cyclodextrin;inclusioncomplex;solubility异槲皮苷（isoquercitrin，IQ）属于黄酮类物质，具有抗炎、抗氧化、降糖、降血压及清除自由基的作用，还具有植物雌激...
