1.Initiation,cargoRecognition2.cargosorting3.cargosorting4.cargosequestration,mVBVesicleformation5.disassemblyescRt-0escRt-IescRt-IIescRt-III(hVps24)Vps/Vta1complexcomplexsubunitsYeastVps27Hse1Vps23Vps28Vps37Mvb12Vps36Vps22Vps25Vps20Snf7Vps24Vps2Vps4Vps60Vta1HumanHrsSTAM1/2Tsg101Vps28Vps37A,B,ChMVB12A,BEAP45EAP30EAP20CHMP6CHMP4A,B,CCHMP3CHMP2A,BSKD1CHMP5LIP5domains/motifsUIM,FYVE,VHS,GATUIM,VHS,SH3,GATUEV,coiled-coil,S-boxcoiled-coilcoiled-coilcoiled-coilGLUE,NZF,WHcoiled-coil,WHPPXY,WHcharged,coiled-coil,MIM2charged,coiled-coil,MIRcharged,coiled-coil,MIRcharged,coiled-coil,MIM1AAA-ATPase,MITcharged,coiled-coil,MIRMITProposedfunctioninteractionwithUb-cargo,PI3P,ESCRT-I(Vps23)Ub-cargointeraction,InteractionwithRsp5Ub-cargoandESCRT-0(Vps27)interactioninteractionwithESCRT-II(Vps36)interactionwithPI3P,Ub-cargo,ESCRT-I(Vps28)interactionwithESCRT-III(Vps20)nucleatorforSnf7oligomerizationhomo-oligomer,cargosequestration,membranedeformationcapforSnf7oligomer,recruitsVps2recruitsVps4forESCRT-IIIdisassemblyinteractsviaMITdomainwithESCRT-III(Vps2)requiredforefficientVps4functionSnapShot:TheESCRTMachineryDavidTeis,1,2SurajSaksena,1andScottD.Emr11CornellUniversity,Ithaca,NY14853,USA,2InnsbruckMedicalUniversity,Innsbruck,AustriaSeeonlineversionforlegendandreferences.182Cell137,April3,2009©2009ElsevierInc.DOI10.1016/j.cell.2009.03.0271.Initiation,cargoRecognition2.cargosorting3.cargosorting4.cargosequestration,mVBVesicleformation5.disassemblyescRt-0escRt-IescRt-IIescRt-III(hVps24)Vps/Vta1complexcomplexsubunitsYeastVps27Hse1Vps23Vps28Vps37Mvb12Vps36Vps22Vps25Vps20Snf7Vps24Vps2Vps4Vps60Vta1HumanHrsSTAM1/2Tsg101Vps28Vps37A,B,ChMVB12A,BEAP45EAP30EAP20CHMP6CHMP4A,B,CCHMP3CHMP2A,BSKD1CHMP5LIP5domains/motifsUIM,FYVE,VHS,GATUIM,VHS,SH3,GATUEV,coiled-coil,S-boxcoiled-coilcoiled-coilcoiled-coilGLUE,NZF,WHcoiled-coil,PPXY,WHcharged,coiled-coil,charged,coiled-coil,charged,coiled-coil,charged,coiled-coil,AAA-ATPase,charged,coiled-MITSnapShot:TheESCRTMachineryDavidTeis,1,2SurajSaksena,1andS...
