生物学实骗技市手册VI.C4目录1.基因组DNA提取.................................................................................................................................91.1.酵母、细菌基因组DNA提取.....................................................................................................................91.2.真核细胞DNA的制备与定量.....................................................................................................................91.3.DNA片段回收与纯化----冻融法..............................................................................................................111.4.琼脂糖凝胶的特点.......................................................................................................................................111.5.琼脂糖核酸电泳..........................................................................................................................................132.聚合酶链式反应（PCR）...................................................................................................................142.1.PCR概述.....................................................................................................................................................142.2.PCR引物设计黄金法则.............................................................................................................................172.3.PCR反应体系.............................................................................................................................................182.4.PCR反应的重要因素.................................................................................................................................192.5.PCR反应条件的选择.................................................................................................................................202.6.PCR常见问题总汇.....................................................................................................................................222.7.减少P...
