ArticleCoordinatedcircRNABiogenesisandFunctionwithNF90/NF110inViralInfectionGraphicalAbstractHighlightsdGenome-widesiRNAscreeningidentifiesimmunefactorsinvolvedincircRNAformationdNF90/NF110promoteback-splicinginthenucleusandalsoarecomponentsofcircRNPsdNuclearexportofNF90/NF110uponviralinfectionleadstoreducedcircRNAlevelsdNF90/NF110releasedfromcircRNPsbindtoviralmRNAsforantiviralimmuneresponseAuthorsXiangLi,Chu-XiaoLiu,WeiXue,...,Run-WenYao,LiYang,Ling-LingChenCorrespondenceliyang@picb.ac.cn(L.Y.),linglingchen@sibcb.ac.cn(L.-L.C.)InBriefLietal.usegenome-widesiRNAscreeningandanefficientcircRNAexpressionreportertoidentifyfactorsinvolvedincircRNAformation.Theyfindthat,uponviralinfection,circRNAbiogenesisandfunctionareregulatedbydouble-strandedRNA-bindingdomaincontainingimmunefactorsNF90/NF110.Lietal.,2017,MolecularCell67,214–227July20,2017ª2017ElsevierInc.http://dx.doi.org/10.1016/j.molcel.2017.05.023MolecularCellArticleCoordinatedcircRNABiogenesisandFunctionwithNF90/NF110inViralInfectionXiangLi,1,4Chu-XiaoLiu,1,4WeiXue,2,4YangZhang,1ShanJiang,1Qing-FeiYin,1JiaWei,2Run-WenYao,1LiYang,2,3,*andLing-LingChen1,3,5,*1StateKeyLaboratoryofMolecularBiologyandShanghaiKeyLaboratoryofMolecularAndrology,CASCenterforExcellenceinMolecularCellScience,ShanghaiInstituteofBiochemistryandCellBiology2KeyLaboratoryofComputationalBiology,CAS-MPGPartnerInstituteforComputationalBiology,ShanghaiInstitutesforBiologicalSciencesUniversityofChineseAcademyofSciences,ChineseAcademyofSciences,320YueyangRoad,Shanghai200031,China3SchoolofLifeScienceandTechnology,ShanghaiTechUniversity,100HaikeRoad,Shanghai201210,China4Theseauthorscontributedequally5LeadContact*Correspondence:liyang@picb.ac.cn(L.Y.),linglingchen@sibcb.ac.cn(L.-L.C.)http://dx.doi.org/10.1016/j.molcel.2017.05.023SUMMARYCircularRNAs(circRNAs)generatedviaback-splicingareenhancedbyflankingcomplementarysequences.ExpressionlevelsofcircRNAsvaryunderdifferentconditions,suggestingparticipationofproteinfac-torsintheirbiogenesis.Usinggenome-wid...
