1903916(1of18)©2019WILEY-VCHVerlagGmbH&Co.KGaA,Weinheimwww.small-journal.comREVIEWProgressinMicrofluidics-BasedExosomeSeparationandDetectionTechnologiesforDiagnosticApplicationsShujingLin,ZixianYu,DiChen,*ZhigangWang,JianminMiao,QichaoLi,DaoyuanZhang,JieSong,andDaxiangCuiDr.S.J.Lin,Z.X.Yu,Prof.D.Chen,Prof.J.M.Miao,Q.C.Li,D.Y.Zhang,Prof.J.Song,Prof.D.X.CuiSchoolofElectronicInformationandElectricalEngineeringShanghaiEngineeringResearchCenterforIntelligentDiagnosisandTreatmentInstrumentKeyLaboratoryforThinFilmandMicrofabricationTechnologyofMinistryofEducationShanghaiJiaoTongUniversityShanghai200240,ChinaE-mail:dchen@sjtu.edu.cnProf.Z.G.WangDepartmentofGeneralSurgeryShanghaiJiaoTongUniversityAffiliatedSixthPeople’sHospitalShanghai200233,ChinaTheORCIDidentificationnumber(s)fortheauthor(s)ofthisarticlecanbefoundunderhttps://doi.org/10.1002/smll.201903916.DOI:10.1002/smll.201903916variouscontents,suchaslipids,mem-braneproteins,cytosolicproteins,andRNA,betweencells.[2–5]Exosomescontainsignalingproteins,RNAandlipidswithinatypeofvesicle.[6,7]Althoughexosomeswereinitiallythoughtofascellulardebris,theyarenowincreasinglyrecognizedasimportantmediatorsthatregulatebiolog-icalbehaviorsofcells.[8]Inrecentyears,exosomeshavebeenconsideredprom-isingbiomarkersfordiagnosisofvariousdiseasesandtreatmentmonitoringindiabeticcardiomyopathy,rheumatoidarthritis,skindiseases,cancer,urinarytractinfection,asthmapathology,andevenorthopedicconditions.[9–18]Moreover,studyofthebioactivityofexosomesincellculturesorinvivohasbecomepopularinanefforttounderstandtheirphysiologicalfunctions.[19–23]Recentstudieshaveshownthatexosomescanbefoundinvariousbodyfluidssuchasblood,semen,plasma,saliva,andurine.[24–28]Thus,separationofexosomesfrombodyfluidsisindispensabletotheexosomeresearchfield.Althoughmuchprogresshasbeenachievedinthefieldofexosomeseparation,therearestillsignificantchal-lengesduetothecomplexnatureofexosome.[29]Todata,severalconventionaltechniqueshavebeenemployedtoseparateexosomes,includingultr...
