CognateNon-LyticInteractionsbetweenCD8+TcellsandBreastCancerCellsInduceCancerStemCell-likeProperties1,2,3RolandG.Stein,1,2StefanEbert,1LauraSchlahsa,4,8ClausJ.Scholz,3,5MatthiasBraun,1,2PetraHauck,1EviHorn,6Camelia-MariaMonoranu,1VincentJ.Thiemann,1MichaelP.Wustrow,1SebastianF.Häusler,1ItsasoMontalbándelBarrio,1,3TanjaN.Stüber,4MatthiasWölfl,1JohannesDietl,6,7AndreasRosenwald,1JoachimE.Diessner,1AchimWöckel,1,2,3JörgWischhusen¹DepartmentofObstetricsandGynecology;2InterdisciplinaryCenterforClinicalResearch(IZKF);3Else-Kröner-Fresenius-ForschungskollegWürzburg(EKFK);4CoreUnitSystemsMedicine,InterdisciplinaryCenterforClinicalResearch,5DepartmentofPediatrics;6InstituteofPathology,and7ComprehensiveCancerCenterMainfranken1-7WürzburgUniversityHospital,UniversityofWürzburg,Würzburg,Germany;8LIMESInstitute,Life&MedicalScienceInstitute,UniversityofBonn,Bonn,Germany.Runningtitle:Tcell-mediateddedifferentiationofbreastcancercellsConflictsofinterest:Noneoftheauthorsisawareofanypotentialconflictofinterestrelatedtothismanuscript.CorrespondingAuthor:JörgWischhusen,PhDWürzburgUniversityHospital,DepartmentofObstetricsandGynecologySectionforExperimentalTumorImmunology,UniversityofWürzburg,Würzburg,GermanyJosef-Schneider-Str.497080WürzburgEmail:Wischhusen_J@ukw.dePhone:0049-931-20125291Research.onJanuary30,2019.©2019AmericanAssociationforCancercancerres.aacrjournals.orgDownloadedfromAuthormanuscriptshavebeenpeerreviewedandacceptedforpublicationbuthavenotyetbeenedited.AuthorManuscriptPublishedOnlineFirstonJanuary28,2019;DOI:10.1158/0008-5472.CAN-18-03871AbstractTargetingoftumorimmuneescapemechanismsholdsenormoustherapeuticpotential.Still,mostpatientsprogressunderimmunecheckpointblockadeandsomeevenbecomehyper-progressors.ToinvestigatehowcancercellsrespondtoactivatedbutineffectiveTcells,wechallengedpeptide-loadedMCF-7breastcancercellswithantigen-specificCD8+Tcellsinwhichlyticgranuleshadbeendestroyedbypre-treatmentwithConcanamycinA.Geneexpressionanalysisaftercoculturer...
