Seeonlineversionforlegendandreferences.SnapShot:EndocyticTraffickingMarnixWieffer,1TanjaMaritzen,1andVolkerHaucke1,21FreieUniversitätandCharitéUniversityMedicineBerlin,2LeibnizInstituteofMolecularPharmacology,Berlin,Germany382Cell137,April17,2009©2009ElsevierInc.DOI10.1016/j.cell.2009.04.012382.e1Cell137,April17,2009©2009ElsevierInc.DOI10.1016/j.cell.2009.04.012SnapShot:EndocyticTraffickingMarnixWieffer,1TanjaMaritzen,1andVolkerHaucke1,21FreieUniversitätandCharitéUniversityMedicineBerlin,2LeibnizInstituteofMolecularPharmacology,Berlin,GermanyMechanismofClathrin-MediatedEndocytosisClathrin-mediatedendocytosisbeginswithcoatnucleationmediatedbycargoandphosphatidylinositol(4,5)-bisphosphate,PI(4,5)P2.Duringthisprocess,cargomol-eculesareclusteredandconcentratedwithintheemergingcoatedpit.Aftercoatassemblyandmembraneinvagination,whichisfacilitatedbyBARdomain-containingproteins,vesiclefissioniscatalyzedbytherecruitmentandhelicalassemblyoftheGTPasedynaminattheneckoftheburgeoningvesicle.MembranescissionrequiresthecooperativeactivityofdynaminwithSH3domain-containingproteins,manyofwhichpossessBARdomainsandregulatetheactincytoskeleton.Actinisrequiredforendocytosisinyeastandmayhavearegulatoryroleinmammaliancells.Theactin-associatedmotorproteinsmyosin1EandmyosinVIarelinkedtotheendocyticmachineryviadynaminorsynaptojaninandDab2,respectively.Duringvesicleuncoating,primaryendocyticvesiclesmayacquireRab5,asmallGTPaseinvolvedintransporttoearlyendosomesandinearlyendosomedockingandfusion.Primaryendocyticvesiclesaretargetedtoandfusewithearlyendosomesexpressingphosphatidylinositol(3)-phosphate,PI(3)P,inaprocessdependentonRab5effectorproteinsandearlyendosomalSNAREs.Differentialsortingofcargodestinedforrecycling(requiringRab11andpossiblyRab4)orforlateendosome/lysosomaldegradation(requiringRab7)canoccuratthelevelofclathrin-coatedpitsandbecom-pletedinearlyendosomalsubdomains.Transportofcargofromearlyendosomestothetrans-Golginetworkrequirestheretromerproteincomplex.PathwaysofEndocytosis...
