Theranostics2017,Vol.7,Issue1http://www.thno.org67TThheerraannoossttiiccss2017;7(1):67-80.doi:10.7150/thno.16752ResearchPaperCD54–NOTCH1axiscontrolstumorinitiationandcancerstemcellfunctionsinhumanprostatecancerChongLi1,2,7*,ShengwuLiu3,5*,RupingYan4*,NingHan6,Kwok-KinWong3,5,LeiLi1,1.DepartmentofUrology,TheFirstAffiliatedHospitalofXi'anJiaotongUniversity,Xi'an710061,China.2.CoreFacilityforProteinResearch,InstituteofBiophysics,ChineseAcademyofSciences,Beijing100101,China.3.DepartmentofMedicine,HarvardMedicalSchool,Boston,MA02115,USA.4.DepartmentofUrology,TheSecondAffiliatedHospitalofKunmingMedicalCollege,Kunming650101,China.5.DepartmentofMedicalOncology,Dana-FarberCancerInstitute,Boston,MA02215,USA.6.DepartmentofLifeScienceandTechnology,ChinaPharmaceuticalUniversity,Nanjing211198,China.7.BeijingJianlanInstituteofMedicine,Beijing100190,China.*contributedequallytothiswork.Correspondingauthor:LeiLI,DepartmentofUrology,TheFirstAffiliatedHospitalofXi'anJiaotongUniversity,Xi'an710061,China;Phone:0086-29-85323661;E-mail:lilydr@163.com.©IvyspringInternationalPublisher.Reproductionispermittedforpersonal,noncommercialuse,providedthatthearticleisinwhole,unmodified,andproperlycited.Seehttp://ivyspring.com/termsfortermsandconditions.Received:2016.07.06;Accepted:2016.09.10;Published:2017.01.01AbstractCancerstemcells(CSCs)areconsideredoneofthekeycontributorstochemoresistanceandtumorrecurrence.Therefore,thepreciseidentificationofreliableCSCmarkersandclarificationoftheintracellularsignalinginvolvedinCSCsremainsagreatchallengeinfieldsrelatingtocancerbiology.Here,weimplementedanovelchemoresistantprostatecancerpatient-derivedxenograft(PDX)modelinNOD/SCIDmiceandidentifiedCD54asacandidategeneamongthemosthighlyenrichedgeneexpressionprofilesinprostatetumorsexposedtochroniccisplatinadministration.AdditionalinvitroandinvivoassaysshowedthatCD54playedacriticalroleintheself-renewalandtumorigenesisofprostateCSCs.Moreover,silencingCD54greatlyreducedthetumorigenesisofprostatecancersbothinv...
