FullTerms&Conditionsofaccessandusecanbefoundathttp://www.tandfonline.com/action/journalInformation?journalCode=kaup20AutophagyISSN:1554-8627(Print)1554-8635(Online)Journalhomepage:http://www.tandfonline.com/loi/kaup20AutophagycontributestosulfonylureaherbicidetoleranceviaGCN2-independentregulationofaminoacidhomeostasisLunZhao,LiDeng,QingZhang,XueJing,MengMa,BinYi,JingWen,ChaozhiMa,JinxingTu,TingdongFu&JinxiongShenTocitethisarticle:LunZhao,LiDeng,QingZhang,XueJing,MengMa,BinYi,JingWen,ChaozhiMa,JinxingTu,TingdongFu&JinxiongShen(2018):AutophagycontributestosulfonylureaherbicidetoleranceviaGCN2-independentregulationofaminoacidhomeostasis,Autophagy,DOI:10.1080/15548627.2017.1407888Tolinktothisarticle:https://doi.org/10.1080/15548627.2017.1407888ViewsupplementarymaterialPublishedonline:29Jan2018.SubmityourarticletothisjournalViewrelatedarticlesViewCrossmarkdataRESEARCHPAPER-BASICSCIENCEAutophagycontributestosulfonylureaherbicidetoleranceviaGCN2-independentregulationofaminoacidhomeostasisLunZhao†,LiDeng†,QingZhang,XueJing,MengMa,BinYi,JingWen,ChaozhiMa,JinxingTu,TingdongFuandJinxiongShen*NationalKeyLaboratoryofCropGeneticImprovement,NationalEngineeringResearchCenterofRapeseed,HuazhongAgriculturalUniversity,Wuhan,ChinaARTICLEHISTORYReceived8December2016Revised9November2017Accepted16November2017ABSTRACTSulfonylurea(SU)herbicidesinhibitbranched-chainaminoacid(BCAA)biosynthesisbytargetingacetolactatesynthase.Plantshaveevolvedtarget-siteresistanceandmetabolictolerancetoSUherbicides;theGCN2(generalcontrolnon-repressible2)pathwayisalsoinvolvedinSUtolerance.Here,wereportanovelSUtolerancemechanism,autophagy,whichwecall‘homeostatictolerance,’isinvolvedinaminoacidsignalinginArabidopsis.TheactivationandreversionofautophagyandGCN2bytheSUherbicidetribenuron-methyl(TM)andexogenousBCAA,respectively,confirmedthatTM-inducedBCAAstarvationisresponsiblefortheactivationofautophagyandGCN2.GeneticandbiochemicalanalysesrevealedalowerproportionoffreeBCAAandmoresensitivephenotypesinatg5,atg7...
