+++KinetochoreMotor-basedmechanismsofspindleassembly++++++++------+SpindlebipolaritySpindlepoleformationChromosomepositioningMolecularmotorsLocalization(inred)ProposedroleRegulatesMTdynamicsandspindlestabilityConnectsspindleMTstochromosomearms;promotesspindlestabilityKinesin-3(+)Klp38B(fly)Kinesin-14(-)Ncd(fly)XCTK2(frog)CHO2(hamster)HSET(human)Kinesin-13(i)Klp10A(fly)Kif2a(frog,mouse,human)Kif2b(human)Kinesin-5(+)Eg5(frog,human)Klp61F(fly)Dynein(-)(fly,frog,mouse,human)Kinesin-4(+)Klp3A(fly)Xklp1(frog)Kinesin-10(+)Nod(fly),Kid(human)Kinesin-7(+)CENP-E(mouse,human)CENP-meta(fly)Kinesin-8(+,i)Klp67A(fly)Kif18A(human)Kinesin-13(i)Klp59C(fly)MCAK(frog,human)MONOPOLARSPINDLEHALFSPINDLELONGERSPINDLELOSSOFCENTROSOMESSPLIT/SPLAYEDPOLESKINETOCHOREMISALIGNMENT,MISORIENTATIONMISALIGNEDCHROMOSOMEARMSBENTSPINDLECrosslinksMTsandslidesantiparallelMTsoutwardSlidesMTspoleward;exertsantagonizingforceagainstKinesin-5DepolymerizesMTsatspindlepolesSlidesMTspoleward;generatescorticalpullingforcePromoteskinetochore-MTattachmentAttacheschromosomearmstospindleandslidestowardcenterSlidesunattachedkinetochoresalongaK-fibertowardspindlecenterDampenskinetochoreoscillationsDepolymerizeskinetochoreMTsInhibitionphenotypesKinesin-4(+)Xklp1(frog),Klp3A(fly)MotormovementMotormovementCellcortexCellcortexChromosomeChromosomeTubulindimersTubulindimersKinetochorefiber(K-fiber)Kinetochorefiber(K-fiber)MTdepolymerizationMTdepolymerizationMicrotubule(MT)Microtubule(MT)CentrosomeCentrosome(+)Plusend-directed(-)Minusend-directed(i)InternalmotordomainSeeonlineversionforlegendandreferences.548Cell134,August8,2008©2008ElsevierInc.DOI10.1016/j.cell.2008.07.038SnapShot:MotorProteinsinSpindleAssemblyRoseLoughlin,BlakeRiggs,andRebeccaHealdMolecularandCellBiologyDepartment,UniversityofCalifornia,Berkeley,Berkeley,CA94720,USA548.e1Cell134,August8,2008©2008ElsevierInc.DOI10.1016/j.cell.2008.07.038SnapShot:MotorProteinsinSpindleAssemblyRoseLoughlin,BlakeRiggs,andRebeccaHealdMolecularandCellBiologyDepartment,Univer...
